肉桂醛对恶性黑素瘤A375细胞增殖和分泌VEGF的影响

目的研究肉桂醛对体外培养的人恶性黑素瘤细胞株A375增殖和分泌血管内皮细胞生长因子(vascularendothelial growth factor,VEGF)的影响。方法 A375细胞在体外培养扩增后,在培养基中分别加入10、20、40μmol/L肉桂醛,分别在培养24、48、72 h MTT法检测细胞活性。同时收集各个浓度肉桂醛处理不同时相的培养液样本,双抗体夹心ELISA法测定其中VEGF分泌量。结果 10μmol/L肉桂醛作用24 h即能抑制A375细胞增殖,10、20、40μmol/L肉桂醛间的抑制强度具有统计学意义(P<0.01)。40μmol/L的肉桂醛作用72 h后细胞增殖抑制率最高,达(88.91±4.5)%。随肉桂醛作用时间延长,A375细胞分泌VEGF的量逐渐减少,72 h后各浓度间的抑制作用具有统计学意义(P<0.01)。40μmol/L肉桂醛作用72 h后A375细胞分泌的VEGF含量最低,为9.55 pg/ml。结论肉桂醛能抑制人黑素瘤细胞增殖和VEGF的分泌,其抑制强度与药物浓度和作用时间成正比。

Cinnamic aldehyde suppresses proliferation and VEGF secretion in human melanoma A375 cells

Objective To investigate the effect of cinnamic aldehyde on the proliferation and secretion of vascular endothelial growth factor(VEGF) in A375 cells.Methods A375 cells were co-cultured with cinnamic aldehyde at the concentrations of 0,10,20 and 40 μmol/L for 24,48 and 72 h respectively.The proliferation of A375 cells was tested by MTT assay.VEGF level in the supernatant was detected by ELISA.Results Cinnamic aldehyde at 10 μmol/L inhibited A375 cells proliferation after 24 hours’ treatment,Cinnamic aldehyde at 10,20 and 40 μmol/L affected the cell proliferation significantly(P<0.01).The proliferation of A375 cells was most remarkably inhibited after co-culture of 40 μmol/L cinnamic aldehyde for 72 h,with the highest inhibitory rate of(88.91±4.5)%.VEGF level in the supernatant was reduced gradually with the elapse of time.After the treatment of 72 h,cinnamic aldehyde at all above concentrations resulted in a significant reduction of VEGF secretion(P<0.01).The lowest level of VEGF in the A375 cells was 9.55 pg/ml after co-culture of 40 μmol/L cinnamic aldehyde for 72 h.Conclusion Cinnamic aldehyde inhibits the proliferation and decreases VEGF secretion in human melanoma cells in a time-and dose-dependent manner.