二草清肝汤对内毒素性肝损害防护作用的实验研究

目的：探讨中药二草清肝汤对内毒素/脂多糖（LPS）性肝损害的防护机制。方法：采用LPS腹腔注射（10mg/kg）制备小鼠内毒素血症肝损害模型,BALB/c小鼠200只随机分为正常对照组、LPS组、二草清肝汤小剂量组和二草清肝汤大剂量组;光镜观察肝组织病理学改变,全自动生化分析仪检测血浆丙氨酸氨基转移酶（ALT）水平,酶联免疫吸附法检测血浆白细胞介素-12（IL-12）、肿瘤坏死因子（TNF）-α浓度,免疫组织化学法检测Toll样受体4（TLR4）表达水平,逆转录聚合酶链反应（RT-PCR）检测肝组织TLR4 mRNA表达水平。结果：二草清肝汤能明显提高小鼠存活率,肝组织病理损害程度减轻;二草清肝汤小剂量组及大剂量组小鼠的IL-12、TNF-α水平显著低于LPS组,差异有显著性意义（均P（0.05）;二草清肝汤小剂量组和大剂量组小鼠肝组织TLR4 mRNA水平也明显低于LPS组,差异均有显著性意义（均P（0.05）,但二草清肝汤大、小剂量组间差异无显著性意义（P〉0.05）。结论：二草清肝汤能明显减轻LPS所致的肝损害,其机制可能与其下调肝脏各种细胞的TLR4表达,同时下调IL-12、TNF-α的水平有关。

Protection mechanisms of Ercao Qinggan Tang against liver injury induced by lipopolysaccharides

Objective:To explore the protective mechanisms of Ercao Qinggan Tang on lipopolysaccharides(LPS) induced liver injury.Methods:The endotoxemia liver injury mice model was produced by intraperitoneal injection with LPS(10mg/kg) and two hundred BALB/c mice were randomly divided into normal control group,LPS group,Ercao Qinggan Tang low dose group and Ercao Qinggan Tang high dose group.The livers of mice were examined for histopathological changes.The levels of aminotransferase(ALT) were measured using automati...