鲨鱼软骨粉(SCP)合剂诱导的抗血吸虫性肝纤维化效应机制的研究

The levelsof hyaluronic acid (HA) and laminin (LN) were detected by radioimmunoassay (RIA) and ELISArespectively probe the mechanism of anti-schistosome egg granuloma and anti-hepaticfibrosis induced by shark cartilage powder (SCP) combining reagent in murineschistosomiasis. Serum HA and LN levels were measured in BALB/c mice with schistosomiasis3 weeks after orally administering SCP combining reagent (7 weeks after infection).Themean serum HA levels in the group with larger dosage of SCP combining reagent (group I)(0.54±0.16mg/L), smaller dosage of SCP combining reagent (group II) (0.91±0.30mg/L ) andsingle SCP (group III) (1.77±0.29mg/L) were significantly lower than that in the group ofsinlge “425” (group IV,2.14±1.22mg/L) and in the infection controls (group V,2.65±0.76mg/L),and the serum HA level in group I was significantly lower than that in group II and IV(P<0.01).But the serum HA level in group IV didn't differ from that in the controlgroup V (P>0.05). The serum LN levels (OD value) in the above mentioned groups from Ito V were 0.70±0.04, 0.84±0.05, 1.05±0.05, 0.84±0.04 and 1.58±0.10 respectively, anda significant difference in the mean serum LN levels in group I to IV was showed incomparison with the level in group V (P<0.01). But no significant difference wasobserved among the group from I to IV (P>0.05). The results suggest that the SCPcombining reagent can decrease the levels of serum HA and LN in host infected withSchistosoma japonicum. The results indicate that the mechanisms of anti-hepatic fibrosiseffects induced by SCP combining reagent is associated with inhibition of HA and LNsecretion.

Studies on the Mechanism of Anti-Schistosome Hepatic Fibrosis Induced by SCP Combining Reagent