乙型肝炎和丙型肝炎患者血清抗肝特异性抗体测定

目的:分析乙型肝炎病毒(hepantitis B virus,HBV)和丙型肝炎病毒(hepantitis C virus,HCV)感染患者血清中肝特异性抗体水平,探讨其与肝细胞损伤的关系。方法:收集2011年1月至2011年10月川北医学院附属医院HBV和HCV感染患者和健康体检者血清,检测患者丙氨酸氨基转移酶(alannine aminotransferase,ALT)和总胆红素水平;通过酶联免疫吸附法检测抗肝细胞膜特异性脂蛋白(liver specific lipoprotein,LSP)和抗去唾液酸糖蛋白受体(asialoglycoprotin receptor,ASGPR)的血清水平;以ALT>40分为肝功能异常组与正常组。使用SPSS13.0统计软件分析结果,P<0.05认为有统计学意义。结果:HBV(H)与健康对照组比较anti-LSP、anti-ASGPR具有统计学差异,HBV组患者anti-ASGPR水平与ALT具有相关性。HCV(H)与健康对照组比较anti-LSP具有统计学差异,HCV组患者anti-LSP与anti-ASGPR具有相关性。HBV患者anti-LSP、anti-ASGPR表达水平较HCV患者高。结论:HBV、HCV感染后,anti-LSP和anti-ASGPR表达高于健康对照组,ALT水平升高时抗肝特异性抗体表达明显增高,表明其可能作为监测肝细胞损伤的特异性免疫学指标。

Expression of the serum liver specific antibodies with HBV and HCV infected

Objective:To analyze the liver specific antibodies expression in serum who with HBV and HCV infected and approach its role.Methods:The samples were collected in Affiliated Hospital of North SiChuan Medical College from January 2011 to October 2011,who with HBV and HCV infected and the healthy check-up as controls,and the ALT and TBIL level be measured at the same time,by ELIAS kit to test anti-LSP and anti-ASGPR expressing.Use SPSS13.0 analysis the results,P<0.05 be viewed as statistically significance.Results:The level of anti-LSP,anti-ASGPR was significance difference between HBV(H) and health control group and there was correlation between anti-ASGPR and ALT in HBV groups.The level of anti-LSP was significance difference between HCV(H) and health control group and there was correlation between anti-LSP and anti-ASGPR in HCV groups.The level of anti-LSP and anti-ASGPR in the groups of HBV expressed more than HCV groups.Conclusions:The level of the anti-LSP and anti-ASGPR was higher in in who with HBV and HCV infected,so they may be help to monitor the injury in hepatocytes with hepatitis virus infected.