| 深低温保存小鼠骨髓细胞及其移植效果 |
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| 引用本文： | 丁振海 张进. 深低温保存小鼠骨髓细胞及其移植效果[J]. 第二军医大学学报, 1989, 10(6): 544-548 |
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| 作者姓名： | 丁振海 张进 |
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| 作者单位： | 第二军医大学放射医学研究室低温生物实验室(丁振海，张进，麦智广，耿怡，余爱国)，第二军医大学放射医学研究室低温生物实验室(杨如俊) |
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| 摘 要： |
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| 关 键 词： | 低温保存 骨髓移植 脾抑素 |
CRYOPRESERVATION OF MURINE BONE MARROW CELLS AND THEIR TRANSPLANTATION |
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| Ding Zhenhai,et al. CRYOPRESERVATION OF MURINE BONE MARROW CELLS AND THEIR TRANSPLANTATION[J]. Former Academic Journal of Second Military Medical University, 1989, 10(6): 544-548 |
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| Authors: | Ding Zhenhai et al |
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| Abstract: | Murine bone marrow cells were cryopreserved in liquid nitrogen with programmable freezing system as a source of hematopoietic cells to transplant into irradiated recipients for the treatment of hematopoietic failure. The results showed that CFU-GM in graft was slightly affected by freezing with a recovery of 75.7%, but CFU-S which act as major role in hematopoietic recons-titution was not obviously injuried (recovery of 126.7%). In the transplantation study, the frozen-thawed bone marrow cells gained survival rate of 43.6% which was slightly higher than obtained by fresh cells, suggesting that cryopreserved bone marrow cells still have intact hematopoietic reconstructive ability. For the purpose of preventing graft-versus-host disease (GVHD), chalone, an extract from calf spleen, was used in our studies and inhibited the early GVHD effectively. |
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| Keywords: | cryopreservation transplantation chalone bone marrow |
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