Time-resolved confocal fluorescence microscopy of porphyrins for phototherapy |
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| Authors: | P. G. Spizzirri J. S. Hill S. B. Kahl K. P. Ghiggino |
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| Affiliation: | (1) School of Chemistry, University of Melbourne, 3052 Parkville, Victoria, Australia;(2) Melbourne Neuroscience Centre, Royal Melbourne Hospital, 3052 Parkville, Victoria, Australia;(3) Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, 94143-0446 San Francisco, CA, USA |
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| Abstract: | The application of a novel time-resolved confocal fluorescence microspectrometer to studies of the distribution and speciation of porphyrin photosensitizers in rat C6 cerebral glioma cells is described. The instrument combines a mode-locked argon ion laser excitation source with time-correlated single photon counting fluorescence detection and has sub-micron spatial and sub-nanosecond temporal resolution. The porphyrins studied were haematoporphyrin derivative (HpD), haematoporphyrin IX (HP), porphyrinc (Pc) and the tetrakiscarborane carboxylate ester of 2,4-( , -dihydroxyethyl) deuteroporphyrin IX (BOPP). From the heterogeneous emission observed in vitro, assignments and spatial location of various porphyrin species are proposed. |
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| Keywords: | Fluorescence Time-resolved confocal microscopy Porphyrins Fluorescence polarization Photodynamic therapy |
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