大鼠肝肠缺血再灌注对远隔器官损伤作用的实验研究

目的 通过动物实验观察肝肠缺血再灌注对远隔器官的损伤作用。方法 健康雄性Wistar大鼠75只,分全肝缺血、肠缺血及肝肠联合缺血3组,分阻断前、阻断末(45 min)、再灌注后0.5、2、6 h共5个时间点,分别活杀5只大鼠取材,测定血浆及组织匀浆中丙二醛和超氧化物歧化酶水平,并观察血尿素氮、血淀粉酶及肌酸激酶等指标的变化。结果 肝肠缺血再灌注后,各组血浆及肺、心、肾、胰组织中丙二醛及超氧化物歧化酶水平均发生显著变化,血尿素氮、血淀粉酶及肌酸激酶混合性同工酶水平均呈上升趋势。结论 肝肠联合缺血再灌注早期可引起机体其他脏器严重损伤;在相同热缺血时间内,肠缺血可能比肝脏缺血引起更大的其他重要脏器损伤。

Multiple organ injury at early stage of intestinal and hepatic ischemia-reperfusion in rats

Objective To investigate the early-stage multiple organ injuries in rats subjected to intestinal and hepatic ischemia-reperfusion. Methods Seventy-five normal male Wistar rats were randomized equally into hepatic ischemia, intestinal ischemia and intestinal-hepatic ischemia groups. Before and at the end of occlusion (45 min), as well as at the time points of 0.5, 2.0 and 6.0 h during the reperfusion, respectively, 5 rats from each group were killed to obtain samples for determination of the contents of malondialdehyde (MDA), superoxide dismutase (SOD) in the blood, lung, kidney, pancreas and heart tissues, along with blood urea nitrogen (BUN), amylase (AMY), and creatine kinase MB (CK-MB). Results The activity of SOD was decreased (P<0.05) and MDA, BUN, AMY and CK-MB levels increased significantly (P<0.05) after ischemia-reperfusion as compared with those before ischemia. Conclusions Intestinal and hepatic ischemia-reperfusion can induce injury of multiple organs at early stage. With the same duration of ischemia-reperfusion, intestinal ischemia may induce severer injury than hepatic ischemia.