原发性肝内胆管结石胆汁和唾液中内源性β－葡萄糖醛酸酶的检测及临床意义

目的探讨内源性β－葡萄糖醛酸酶（β－glucuronidase，β－G）活性在原发性肝内胆管结石形成中的作用和意义。方法采用改良的Fishman方法对原发性肝内胆管结石患者胆汁、唾液中内源性β－G进行测定，分析内源性β－G活性变化与肝内胆管结石的关系。结果无论术前及术后，肝内胆管结石患者胆汁和唾液中内源性β－G活性均明显高于对照组（P〈0．05）；与术前相比，术后2周胆汁和唾液中内源性β－G水平均较术前降低，但是只有胆汁内源性β－G水平变化有统计学意义（P〈0．05）。结论内源性β－G可能与原发性肝内胆管结石的成石有关。

Significance of Endogenous β-glucuronidase Test in Bile and Saliva in Primary Intrahepatic Bile Duct Stone

Objective To investigate the role of endogenous β-glucuronidase in the formation of primary intrahepatic bile duct stone. Methods The activity endogenous of β-glucuronidase in the bile and saliva was tested by modified Fishman method, and comparison and metastasis of the relation between endogenous β-glucuronidase and bile duct stone patterns were performed. Results Expression levels of endogenous β-glucuronidase of bile and saliva in primary intrahepatic bile duct stone group were both higher than in the control group either pre-operation or post-operation （P〈0.05）. Bile endogenous β-glucuronidase, instead of saliva endogenous β-glucuronidase, was statistically higher pre-operation than post-operation. Conclusion Endogenous β-glucuronidase may play a role in the formation of primary intrahepatic bile duct stone.