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绞股蓝多糖的提取纯化和含量测定
引用本文:宋淑亮,唐金宝,吉爱国,梁浩,朱鹏,王伟莉. 绞股蓝多糖的提取纯化和含量测定[J]. 中药材, 2006, 29(6): 595-598
作者姓名:宋淑亮  唐金宝  吉爱国  梁浩  朱鹏  王伟莉
作者单位:1. 山东大学药学院,山东济南250012;山大威海生物技术研发中心,山东威海264209
2. 潍坊医学院药学专业,山东潍坊261042
3. 山大威海生物技术研发中心,山东威海264209
4. 山东大学药学院,山东济南250012
摘    要:通过正交设计实验,综合考虑提取效率和成本,优化了绞股蓝多糖的提取方法,并对绞股粗多糖进行了初步纯化,测定了提取物的糖含量,其含量水平较前有明显提高,这将为绞股蓝进一步开发生产奠定基础。

关 键 词:绞股蓝  多糖  正交设计  提取  纯化  含量测定
文章编号:1001-4454(2006)06-0595-04
收稿时间:2005-11-24
修稿时间:2005-11-24

The Extraction,Purification and Assaying of Gynostemma pentaphyllum Polysaccharides
SONG Shu-liang,TANG Jin-bao,JI Ai-guo,LIANG Hao,ZHU Peng,WANG Wei-li. The Extraction,Purification and Assaying of Gynostemma pentaphyllum Polysaccharides[J]. Journal of Chinese medicinal materials, 2006, 29(6): 595-598
Authors:SONG Shu-liang  TANG Jin-bao  JI Ai-guo  LIANG Hao  ZHU Peng  WANG Wei-li
Affiliation:Pharmacy College of Shandong University, Jinan 250012, China.
Abstract:By orthogonal design, and considering extracting efficiency and cost, optimizing the extract method of Gynostemma pentaphyllum polysaccharides. We purified the crude Gynostemma pentaphyllum polysaccharides initially, and assayed the polysaccharides content of Gynostemma pentaphyllum polysccharides. The content of Gynostemma pentaphyllum polysaccharides was sigificantly higher than the predecessor. It would provide conditions for the deep exploitation of Gynostemma pentaphyllum.
Keywords:Gynostemma pentaphyllum  Polysaccharides  Orthogonal design  Extraction  Purification  Assaying
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