| 荧光定量PCR对N0期结直肠癌淋巴结微转移的检测及其临床意义 |
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| 引用本文： | 陈维香,费绍华,祝蕾,伍建,丁君,陶祥. 荧光定量PCR对N0期结直肠癌淋巴结微转移的检测及其临床意义[J]. 肿瘤防治研究, 2008, 35(8): 583-585. DOI: 10.3971/j.issn.1000-8578.830 |
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| 作者姓名： | 陈维香 费绍华 祝蕾 伍建 丁君 陶祥 |
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| 作者单位： | 上海市公利医院病理科; 复旦大学附属肿瘤医院分子病理室; |
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| 基金项目： | 上海市浦东新区青年基金资助项目(PW2005B-1) |
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| 摘 要： | 目的探讨N0期大肠癌淋巴结微转移的检测及其临床意义。方法采用荧光定量聚合酶链反应(FQ-PCR)检测45例行根治术的N0期大肠癌患者的453枚淋巴结中细胞角蛋白20(cytokeratin,CK20)mRNA的表达以检出微转移。结果45例N0期大肠癌病人的453枚淋巴结中,有20例(44.4%)共46枚(10.2%)淋巴结检出微转移。微转移与患者年龄、性别、肿瘤大小、分化程度等无关,但与肿瘤浸润深度相关(χ2=5.445,P<0.05)。20例微转移患者与25例无微转移患者平均随访时间分别为19.6和21.4月。20例微转移患者中7例发生复发转移,5例死亡;而无微转移25例中仅1例因复发转移而死亡(χ2=7.305,P<0.05)。有微转移组和无微转移组的生存率分别是75.0%(15/20)和96.0%(24/25),两组患者之间差异有统计学意义(χ2=4.240,P<0.05)。结论CK20mRNA的FQ-PCR是检测N0期大肠癌淋巴结微转移灵敏而特异的方法,可对精确临床分期、判断患者预后及制定合理的治疗方案提供一定的理论依据。
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| 关 键 词： | 结肠直肠肿瘤 淋巴转移 微转移 角蛋白 荧光定量聚合酶链反应 |
| 收稿时间： | 2007-08-08 |
| 修稿时间： | 2008-03-25 |
Detection of Micrometastasis and Its Clinical Significance in Lymph Nodes from N0 Colorectal Cancers by FQ-PCR |
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| CHEN Wei-xiang,FEI Shao-hua,ZHU Lei ,WU Jian ,DING Jun ,TAO Xiang. Detection of Micrometastasis and Its Clinical Significance in Lymph Nodes from N0 Colorectal Cancers by FQ-PCR[J]. Cancer Research on Prevention and Treatment, 2008, 35(8): 583-585. DOI: 10.3971/j.issn.1000-8578.830 |
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| Authors: | CHEN Wei-xiang FEI Shao-hua ZHU Lei WU Jian DING Jun TAO Xiang |
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| Affiliation: | 1.Department of Pathology; Gongli Hospital; Shanghai 200135; China; 2.Department of Molecular Pathology; Cancer Hospital; Fudan University; |
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| Abstract: | Objective To study the detection of lymph node micrometastasis in N0 colorectal cancer patients and its clinical significance in these patients.Methods In the study,453 lymph nodes obtained from 45cases of N0 colorectal cancer going curative operation were examined,using fluorescent quantity polymerase chain reaction(FQ-PCR) assay to detect cytokeratin 20(CK20) mRNA expression for identifying micrometastasis.Results Micrometastasis was detected in 46 lymph nodes(10.2%,46/453) of 20 cases(44.4%,20/45).The pr... |
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| Keywords: | Colorectal neoplasms Lymphatic metastases Micrometastasis Cytokeratin Fluorescent quantity Polymerase Chain Reaction(FQ-PCR) |
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