小细胞肺癌患者外周血中Th9细胞水平检测及其临床意义

目的: 检测小细胞肺癌(SCLC)患者血清中白细胞介素9(IL-9)的水平和外周血中Th9的细胞比例,探讨Th9在SCLC疾病进展过程中的作用。方法: 选择20例局限期SCLC患者(局限期SCLC组)、16例扩散期SCLC患者(扩散期SCLC组)和10名健康对照者(健康对照组)。ELISA法检测各组研究对象血清中IL-9水平,流式细胞术检测各组研究对象外周血Th9细胞在CD4⁺T细胞中所占比例。结果: SCLC组患者外周血中Th9细胞比例高于健康对照组(t=3.731,P<0.01),局限期SCLC患者外周血中Th9细胞比例低于扩散期SCLC组(t=2.825,P<0.01)。SCLC组患者血清IL-9水平高于健康对照组(t=4.001,P<0.01),局限期SCLC组患者血清中IL-9水平低于扩散期SCLC组(t=3.089,P<0.01);结论: Th9可能通过分泌IL-9直接促进SCLC的疾病进展,外周血中Th9细胞和IL-9可能成为评价小细胞肺癌的分子标志。

Detection of Th9 cells in peripheral blood of patients with small cell lung cancer and its clinical significance

Objective: To detect the ratios of peripheral blood Th9 cells and the levels of serum IL-9 in the patients with small cell lung cancer (SCLC),and to investigate the effect of Th9 cells on the development of human SCLC. Methods: Twenty SCLC patients at limited stage(limited stage SCLC group),16 SCLC patients at extensive stage(extensive stage SCLC group), and 10 healthy controls (healthy control group) were selected.The ratios of Th9 cells to CD4⁺ cells in peripheral blood and the levels of serum IL-9 were detected by flow cytometry and ELISA, respectively. Results: Compared with healthy control group,the level of serum IL-9 of the patients in SCLC group was increased(t=4.001,P<0.01),and the levels of IL-9 of the patients in limited stage SCLC group was lower than that in extensive stage SCLC group(t=3.089,P<0.01).The ratio of Th9 cells of the patients in SCLC group was higher than that in healthy control group (t=3.731,P<0.01),and the ratio of Th9 cells of the patients in limited stage SCLC group was lower than that in extensive stage SCLC group (t=2.825,P<0.01). Conclusion: Th9 might directly promote the development of SCLC through secreting IL-9.The peripheral blood Th9 cells and IL-9 might be biomarkers to evaluate SCLC progression.