枳实总黄酮苷对功能性消化不良模型大鼠内脏敏感性的影响

目的探讨枳实总黄酮苷对功能性消化不良(FD)模型大鼠内脏敏感性的影响及可能的作用机制。方法 10日龄雄性SD大鼠40只随机分为正常组,模型组,枳实总黄酮苷高、中、低剂量组,每组8只。采用碘乙酰胺灌胃联合夹尾刺激的方法,建立FD大鼠模型。大鼠8周龄时,开始灌胃相应药物,共14 d。检测胃扩张刺激时腹部撤退反射(AWR)评分、颈斜方肌肌电(EMG)及胃顺应性,评价枳实总黄酮苷对大鼠内脏敏感性的影响,并筛选出最优剂量进行下一步研究。将另15只10日龄SD大鼠随机分为正常组、模型组、枳实总黄酮苷最优剂量组,每组5只。造模方法及干预方法同上。检测大鼠胃及脊髓中5-羟色胺(5-HT)含量及脊髓中c-fos mRNA的表达。结果大鼠胃内球囊压力在40、50、60 mm Hg时,枳实总黄酮苷高、中剂量组AWR评分及颈斜方肌EMG均方根变化率明显低于模型组(P0.05),胃顺应性高于模型组(P0.05),而枳实总黄酮苷中、高剂量组间比较无统计学差异(P0.05)。因此,枳实总黄酮苷中剂量为最优剂量。枳实总黄酮苷中剂量可明显减低5-HT的含量、c-fos mRNA表达(P0.05),且与正常组比较无统计学差异(P0.05)。结论枳实总黄酮苷可以改善FD模型大鼠内脏高敏感性,作用机制可能与调节5-HT及c-fos表达有关。

Effect of total flavonoid glycosides from Fructus Aurantii Immaturus on visceral hypersensitivity in rats with functional dyspepsia

Objective To investigate the effect and mechanism of total flavonoid glycosides from Fructus Aurantii Immaturus (Immature Bitter Orange,Zhishi)on visceral hypersensitivity in rats with functional dyspepsia (FD).Methods Forty ten-day-old male SD rats were randomly divided into five groups:nor-mal group,model group,total flavonoid glycosides of Zhishi(TFGZ)high-dose group,mid-dose group and low-dose group (each n =8).FD model rats were induced by intragatrical administration of iodoacet-amide with tail-clamping stress.When the rats were 8 weeks old,they were intragstrically administered corresponding dosage of TFGZ for consecutive 14 days.Then abdominal withdrawal reflex (AWR)score, electromyographic (EMG)activity of Musculus trapezius cervicalis and gastric accommodation during gas-tric balloon distention were recorded.The optimal dose of TFGZ was screened for the next study.Fifteen more ten-day-old male SD rats were randomly divided into three groups—normal group,model group and TFGZ group at optimal dosage.The method of modeling and treatment were as same as before.After treatment,the expression of 5-HT in gastric and spinal cord tissue and c-fos mRNA in spinal cord tissue were measured.Results Under the intragastric balloon pressure of 40,50 and 60 mmHg,AWR score and change rate of root-mean-square (RMS)of EMG of rats in TFGZ high-dose group and mid-dose group were significantly lower than those of model group (P <0.05),while gastric accommodation was higher than that of model group (P <0.05).While there was no significant difference between the mid-dose group and high-dose group (P >0.05),so middle dose was confirmed to be optimal.TFGZ middle dose significantly decreased 5-HT and c-fos mRNA expression of rats with FD (P <0.05),and there was no significant difference with those in normal group (P >0.05).Conclusion Total flavonoid glycosides from Zhishi could effectively reduce the visceral hypersensitivity in rats with functional dyspepsia.Its mechanism may through the way of lowering the level of 5-HT and c-fos in gastric and spinal cord tissue.