富血小板血浆对创伤性颅脑损伤大鼠神经功能的保护作用

目的：探讨富血小板血浆（PRP）对创伤性颅脑损伤（TBI）大鼠神经功能的保护作用，阐明其作用机制。方法：60只SD大鼠随机分为假手术组（仅开放骨窗）、TBI模型组（TBI组）和TBI模型并PRP处理组（PRP组），每组20只。PRP组大鼠于术后当天、第2和6天血管内注射PRP，TBI组和假手术组大鼠注射生理盐水。3组大鼠分别于术后第1、3和7天时行改良大鼠神经功能缺损（mNSS）评分；每组各取10只大鼠处死并取脑，制作切片行组织学观察，剩余大鼠行Morris水迷宫实验。结果：与假手术组比较，TBI组和PRP组大鼠mNSS评分明显升高（P<0.05）；与TBI组比较，PRP组大鼠mNSS评分明显降低（P<0.05）；与TBI组比较，PRP组大鼠脑组织损伤体积明显减小（P<0.05）；Nissl染色，PRP组大鼠脑损伤区细胞排列相对整齐，新生毛细血管多于TBI组；免疫组织化学染色，与假手术组比较，TBI组大鼠脑损伤区域有较多胶质纤维酸性蛋白阳性（GFAP⁺）细胞，但神经元核抗原阳性（neuN⁺）细胞少于PRP组（P<0.05）。Morris水迷宫实验，PRP组大鼠在各象限逃避潜伏期均低于TBI组（P<0.05），穿越平台次数及第三象限游泳时间高于TBI组（P<0.05）。结论：PRP对TBI大鼠可发挥神经保护作用。

Protective effect of platelet-rich plasma on never function in rats with traumatic brain injury

Objective:To study the protective effect of platelet-rich plasma on never function in the rats with traumatic brain injury (TBI),and to clarify its mechanisms. Methods:Sixty SD rats were randomly divided into sham group (opened skull bone window only),TBI group and platelet-rich plasma treatment group (PRP group) (n=20).The rats in PRP group were injected with platelet-rich plasma through vessel on the 1st day,the 2nd day and the 6th day after operation while the rats in sham group and TBI group were treated with saline at the same time.The neurological function defects were assessed with modified neurological severity score (mNSS)on the 1st, 3rd and 7th after operation.Then 10 rats were taken from each group and executed,and the brain tissues were taken.The brain sections were prepared for the histological observation and the others of each group were tested with Morris water maze.Results:Compared with sham group,the mNSS scores of the rats in TBI and PRP group were increased (P <0.05);the mNSS score of the rats in PRP group was decreased compared with TBI group (P <0.05).The injured volume of rat brain tissue was reduced significantly in PRP treated group compared with TBI group (P <0.05).The Nissl staining results showed that the injury area in PRP group had a more neat rows and a larger number of new blood vessels compared with TBI group.The immunohistochemical staining results showed the injured area had a higher level expression of GFAP+ cells in TBI group compared with PRP group,but the amount of neuN+ cells was smaller than that in PRP group (P <0.05).The Morris water maze test results showed that there were a shorter escape latency time,more times acrossing platform and a larger swimming time during platform quadrant in PRP group compared with TBI group (P < 0.05).Conclusion:Platelet-rich plasma has a significant role in protecting the neurological function of TBI rats.