电针对脊髓损伤大鼠 Nogo/NgR 信号通路相关因子基因表达的影响

目的观察电针对脊髓损伤大鼠Nogo/NgR信号通路相关因子基因表达变化,探讨电针治疗脊髓损伤的可能作用机制。方法大鼠随机分为模型组、电针组、阻断剂组、电针+阻断剂组、假手术组,用改良Allen’s撞击法制备T10脊髓损伤模型,分别于治疗后1、7、14 d对各组大鼠进行BBB功能评分;在7、14 d于损伤处提取脊髓组织,以蛋白印迹法(Western blot)测定各组大鼠脊髓组织中Nogo-A、NgR、LINGO-1蛋白的表达变化,实时荧光定量PCR测定Nogo-A、NgR、LINGO-1 mRNA的表达变化。结果脊髓损伤后,造模大鼠1 d后BBB评分均为0分;治疗7、14 d后,各治疗组BBB评分均明显高于模型组(P0.05),且电针+阻断剂组优于电针组、阻断剂组(P0.05)。与假手术组比较,同时间点模型组各基因表达均明显提高(P0.05);与模型组比较,各治疗组基因表达明显下降(P0.05);电针+阻断剂阻组与电针组比较,同时间点LINGO-1基因表达有显著差异(P0.05)。结论 Nogo/NgR信号通路在脊髓损伤后神经的再生与修复过程中起着关键作用,电针通过抑制Nogo/NgR信号通路而对脊髓损伤大鼠起治疗作用。

Effect of electro-acupuncture on Nogo/NgR signal pathway related fac-tors in spinal cord injury rats

Objective To observe the effect of electro-acupuncture on Nogo /NgR signal pathway related factor gene expression in rats with spinal cord injury,so as to investigate the potential mechanism of elec-tro-acupuncture(EA)treatment for spinal cord injury.Methods The rats were randomly divided into model group,EA group,blocking agent group,EA +blocking agent group,and sham operation group. T10 spinal cord injury model was induced by the modified Allen’s impact method,and the BBB function score on day 1,7,and 14 in each group were obtained after treatment respectively.Spinal cord tissues were extracted on day 7 and 14,then Nogo-A,NgR and LINGO-1 protein expression in spinal cord tis-sues of rats were detected by Western blot.The mRNA expression of LINGO-1,NgR and Nogo-A were measured by real-time fluorescence quantitative PCR.Results After spinal cord injury,BBB scores of model rats was 0 on day 1;after 7 or 14 days of intervention,the BBB scores of each treatment group were significantly higher than those of model group(P <0.05),and the EA +blocking agent group was better than the EA group and blocking agent group(P <0.05).Compared with sham operation group at the same time point,the expression of each gene in model group significantly improved(P <0.05 ). Compared with the model group,the gene expression in each treatment group significantly decreased(P <0.05),at the same time,there was a significant difference in LINGO-1 gene expression between the EA+blocking agent group and the EA group(P <0.05).Conclusion Nogo /NgR signal pathway plays a key role in the process of nerve regeneration and repairment after spinal cord injury.Electro-acupuncture may be via inhibiting Nogo /NgR,LINGO-1 signal pathway to treat spinal cord injury in rats.