CD14和IL-8基因多态性与新生儿坏死性小肠结肠炎易感性的关联性分析

目的：探讨白细胞分化抗原14（CD14）-159C/T（rs2569190）和白细胞介素8（IL-8）-251A/T（rs4073）基因多态性与坏死性小肠结肠炎（NEC）易感性之间的关系，阐明NEC易感性的可能影响因素，为NEC的发病机制研究提供遗传学理论依据。方法：选取28例NEC新生儿（NEC组）和41例非NEC新生儿（对照组）作为研究对象，提取并应用基因聚合酶链式反应（PCR）扩增其外周血DNA，并采用Sanger基因测序方法，检测CD14-159C/T和IL-8-251A/T区域的等位基因频数和基因型频数的分布，探讨其与NEC易感性的关联性。结果：CD14-159C/T和IL-8-251A/T位点基因型频数分布符合Hardy-Weinberg平衡定律（P>0.05）；CD14-159C/T的等位基因和基因型频数分布在2组间比较差异无统计学意义（P>0.05）；IL-8-251A/T位点的基因型频数分布在2组间比较差异无统计学意义（P>0.05）；而NEC组IL8-251A/T基因位点T等位基因频数分布高于对照组（χ²=4.184，P=0.041，OR=2.14，95% CI：1.03~4.46）。结论：CD14-159C/T的基因位点多态性和NEC的发病无关联，而IL-8基因的-251 T位点与NEC发病易感性存在关联，IL-8基因的-251 T等位基因突变可能是NEC的危险因素之一。

Analysis on association between polymorphism of CD14 and IL-8 gene and susceptibility of necrotizing enterocolitis

Objective:To investigate the relationship between the gene polymorphism cluster of differentiation 14 (CD14)-159C/T (rs2569190),and interleukin-8 (IL-8)-251A/ T (rs4073)and the susceptibility of necrotizing enterocolitis (NEC),to clarify the influencing factors of susceptibility of NEC and to provide genetics theory basis for the research on the pathogenesis of NEC. Methods:Total 28 newborns with NEC and 41 newborns without NEC were selected.The amplification of peripheral blood DNA was conducted by PCR.The genotypic and allelic frequencies of CD14-159C/T and IL-8-251A/T of the patients were detected by Sanger DNA sequencing method. The relationship between them and the susceptibility of NEC was studied.Results:The distribution of genotypic frequencies of CD14-159C/T and IL-8-251A/T was consistent with Hardy-Weinberg equilibrium (P >0.05).There were no significant differences of the allelic and genotypic frequencies of CD14-159C/T,or genotypic frequencies of IL-8-251A/T between two groups (P >0.05).While in NEC group,the T allelic frequency of IL-8-251A/T site was higher than that in control group (χ2 = 4.184, P = 0.041, OR = 2.14, 95% CI: 1.03 - 4.46 ). Conclusion:The polymorphism of CD14-159C/T is irrelevant to the pathogeny of NEC,but T allelic frequency of IL-8-251A/T site might be related to the susceptibility of NEC.So T allele in IL-8-251A/T may be one of the danger factors of NEC.