miR-139-5p在小细胞肺癌组织中的表达及其临床意义

目的：探讨miR-139-5p在小细胞肺癌组织中的表达及其临床意义，阐明miR-139-5p在小细胞肺癌发生发展中的作用。方法：采用细胞生长、凋亡及周期实验检测miR-139-5p的生物学功能；实时荧光定量PCR法检测50例小细胞肺癌患者癌组织及癌旁正常组织中miR-139-5p的表达，结合临床资料分析其在临床中的作用。结果：miR-139-5p在小细胞肺癌组织中的表达水平低于正常肺组织（P<0.01）；转染miR-139-5p mimics后能够明显上调细胞中miR-139-5p的表达，差异有统计学意义（P<0.01）。与对照组比较，上调miR-139-5p的表达后细胞的增殖能力降低（P<0.01），G₁期细胞明显增多（P<0.05），细胞周期发生G₀/G₁期阻滞。单因素分析，miR-139-5p的表达与患者的性别和年龄无关（P>0.05），miR-139-5p在局限期（LD）患者中的表达较广泛期（ED）患者低，两者的miR-139-5p表达率比较差异有统计学意义（χ²=10.546，P=0.001）；miR-139-5p在耐药患者中的表达较化疗敏感患者增高（χ²=7.025，P=0.008）。miR-139-5p在存活患者中的表达较死亡患者降低（χ²=7.697，P=0.006）；Cox回归分析，疾病分期和miR-139-5p的表达可作为小细胞肺癌独立的预后因子。结论：miR-139-5p通过抑制细胞增殖、促进细胞凋亡及诱导细胞周期阻滞参与调节小细胞肺癌发生发展，可作为评估小细胞肺癌患者临床预后的靶基因。

Expression of miR-139-5p in small cell lung cancer tissue and its clinical significance

Objective:To explore the expression of miR-139-5p in small cell lung cancer (SCLC)tissue and its clinical significance, and to clarify the role of miR-139-5p in the occurrence and development of SCLC. Methods:The biological function of miR-139-5p was examined by cell growth,apoptosis and cell cycle analysis. The expressions of miR-139-5p in 50 cases of cancer tissue and paracarcinoma normal tissue were examined by QRT-PCR.Combined with the clinical data,the role of miR-139-5p in clinic was anzlyzed.Results:The expression level of miR-139-5p in SCLC tumor tissue was lower than that in normal lung tissue (P <0.01).The expression level of miR-139-5p in the cells was un-regulated significantly after transferred miR-139-5p mimics (P <0.01).Compared with control group,the proliferation abilitiy of cells was reduced after up-regulating the expression of miR-139-5p (P < 0.01),the number of cells at G1 phase was increased (P < 0.05),and the cells were arrested at G0/G1 phase.The miR-139-5p expression was not associated with gender and age (P > 0.05).The expression level of miR-139-5p in the patient at LD stage was lower than that of the patients at ED stage (P <0.01).The expression level of miR-139-5p in the resistant patients was higher than that of the patients sensitive to chemotherappy (P <0.01).The expression level of miR-139-5p in the survival patients was lower than that in the death patients (P <0.01).Cox regression analysis indicated that miR-139-5p expression and disease stage were the independent prognostic factors for SCLC.Conclusion:miR-139-5p in participates in the occurrence and development of SCLC by inhibiting the cell proliferation,promoting apoptosis and inducing the cell cycle arrest;it may be used as a target gene to evaluate the prognosis of SCLC patients.