还原型谷胱甘肽对小鼠放射性肺损伤的影响

目的 研究还原型谷胱甘肽(GSH)对放射性肺损伤(RILI)小鼠的作用及其损伤机制。方法 100只BALB/c小鼠随机数字表法分为健康对照组、15 Gy组、30 Gy组、15 Gy+GSH组和30 Gy+GSH组,每组20只。GSH组小鼠腹腔注射0.2 ml 240 mg/ml GSH,单纯照射组腹腔注射等体积的生理盐水作对照,建立15和30 Gy X射线的RILI模型。于照射前、照射后1、2、3周收集血样,采用ELISA法检测MMP-9、TIMP-1、IL-4和IL-6的表达量,HE染色观察病理情况,并分析小鼠的体重和免疫系统变化。结果 照射后,小鼠的脾脏指数先急速下降,下降速度随照射剂量增加而降低(F=29.84,P<0.05);1周后随照射剂增加而慢慢上升(F=13.91、4.61,P<0.05)。血清MMP-9与IL-6表达随照射剂量增加而升高(F=81.27、10.86,P<0.05),TIMP-1和IL-4相反。随照射后时间延长,MMP-9表达量先上升后下降(F=52.22,P<0.05),TIMP-1和IL-4先下降后上升(F=138.96、8.48,P<0.05),IL-6表达始终上升。结论 GSH具有抗放射性肺损伤作用,尤其在低剂量照射下更明显。

Effect of reduced glutathione tablets on radiation-induced lung injury in mice

Objective To study the effects and underlying mechanism of reduced glutathione (GSH) tablets on radiation-induced lung injury in mice. Methods A total of 100 BALB/c mice were randomly divided into 5 groups: 15 Gy, 15 Gy+GSH, 30 Gy, 30 Gy+GSH, and healthy control group. Mice of GSH groups were intraperitoneally injected with 0.2 ml of 240 mg/ml GSH, and other two radiation alone groups were treated with an equal volume of saline. The mice were delivered with 15 Gy (30 Gy) of X-rays to build a RILI model. At 0, 1, 2, 3 weeks after irradiation, blood samples were collected for ELISA measurements of MMP-9, TIMP-1, IL-4 and IL-6, the lung pathology was detected by HE, and the mouse weight and immune system were also analyzed. Results The mice spleen index fell sharply after irradiation and the decline rate decreased with increasing dose (F=29.84, P<0.05), then this index slowly increased at 1 week after irradiation along with radiation dose (F=13.91, 4.61, P<0.05). The expressions of MMP-9 and IL-6 in blood serum increased along with radiation dose (F=81.27, 10.86, P<0.05), but those of TIMP-1 and IL-4 decreased. In addition, following irradiation, the expression of MMP-9 increased at first and then decreased (F=52.22, P<0.05), while the expressions of TIMP-1 and IL-4 dropped immediately at first, and then increased until at 3 week after radiation(F=138.96, 8.48, P<0.05), and the expression of IL-6 gradually increased. Conclusions GSH has significant radioresistant effect on RILI, especially at low dose.