降压宝蓝片含药血清对过氧化氢损伤的人脐静脉内皮细胞的保护作用

目的:探讨降压宝蓝片含药血清对过氧化氢(H₂O₂)诱导人脐静脉血管内皮细胞(HUVECs)损伤的保护作用。方法:体外培养HUVECs,分为空白组、模型组、降压宝蓝片含药血清低、中、高浓度组(含量血清体积分数为5%,10%,15%),显微镜下观察细胞形态,用噻唑蓝(MTT)法检测HUVECs活力,微板法检测上清液中乳酸脱氢酶(LDH)的活性及一氧化氮(NO)的含量,ELISA法检测内皮素-1(ET-1),组织型纤溶酶原激活物(tPA),组织型纤溶酶原激活物抑制剂(PAI-1)的含量。结果:与空白组比较,模型组细胞活力显著降低,上清液中LDH活性明显增高,ET-1和PAI-1含量显著增加,NO和tPA含量显著下降(P<0.01);与模型组比较,降压宝蓝片中、高浓度组细胞活力显著升高,LDH活性显著减低,ET-1和PAI-1含量显著减少,NO和tPA显著升高(P<0.05,P<0.01)。结论:降压宝蓝片含药血清对H₂O₂诱导的HUVECs损伤具有显著地保护作用。

Protective Effect of Medicated Serum Prepared with Jiangya Baolan Tablets on Human Umbilical Vein Endothelial Cells Injured by Hydrogen Peroxide

Objective: To investigate the protective effect of medicated serum prepared with Jiangya Baolan tablets on the human umbilical veinendothelial cells (HUVECs) injured by hydrogen peroxide. Method: HUVECs were cultured in vitro and used for experiment. They were divided into five groups as follows:blank group, model group, Jiangya Baolan tablets low dosage group, mid-dosage group and high-dosage group (volume fraction of 5%, 10%, 15% respectively). The morphological change of the cells was observed under microscope. MTT was used to determine the HUVECs viability. The levels of lactate dehydrogenase (LDH), and nitric oxide (NO) in supernatant were measured by the microplate method;the levels of endothelin-1(ET-1), tissue plasminogen activator(tPA) and plasminogen activator inhibitor-1(PAI-1) were determined by enzyme-linked immunosorbent assay (ELISA). Result: Compared with the blank group, the model group significantly decreased HUVECs viability, significantly increased the levels of LDH in supernatant, significantly increased ET-1 and PAI-1, and significantly decreased the levels of NO and tPA (P<0.01). Compared with the model group, Jiangya Baolan tablets mid-dosage and high-dosage groups significantly increased HUVECs viability, significantly decreased the levels of LDH, ET-1, PAI-1, and significantly increased the levels of NO and tPA (P<0.05,P<0.01). Conclusion: Medicated serum prepared with Jiangya Baolan tablets has significant protective effect on HUVECs injured by H₂O₂.