养血化瘀消癥汤对大鼠子宫内膜异位病灶的抑制作用和机制

目的:研究养血化瘀消癥汤对大鼠子宫内膜异位病灶的抑制作用及作用机制。方法:取雌性SD大鼠,除正常组10只大鼠外,采用自体移植法建立子宫内膜异位症大鼠模型,造模成功的大鼠随机分为模型组,养血化瘀消癥汤高、中、低剂量组(12,8,4 g·kg^-1)和孕三烯酮组(0.05 g·kg^-1),每组10只。分别ig给药28 d后,采集病灶组织标本,观察养血化瘀消癥汤对大鼠子宫内膜异位组织体积的抑制作用;RT-qPCR和Western blot检测各组大鼠异位组织血管内皮生长因子(vascular endothelial growth factor,VEGF)和血管内皮生长因子受体-1(VEGFR-1)的表达。结果:与正常组比较,模型组大鼠子宫内膜异位组织VEGF,VEGFR-1 mRNA及蛋白表达明显升高(P<0.05);与模型组比较,药物组大鼠的子宫内膜异位组织较模型组明显抑制,各给药组大鼠的子宫内膜异位组织VEGF和VEGFR-1 mRNA及蛋白表达表达均明显低于模型组(P<0.05)。结论:养血化瘀消癥汤能明显抑制模型大鼠子宫内膜异位组织的发展,这种抑制作用可能与其抑制VEGF/VEGFR信号通路调控病灶血管生成有关。

Effect and Mechanism of Yangxue Huayu Xiaozheng Decoction on Formation of Endometriosis Foci in Rats

Objective: To study the effect and mechanism of Yangxue Huayu Xiaozheng decoction on formation of endometriosis foci in rats. Method: Female SD rats were selected and all the other rats except 10 rats in normal group were used to establish endometriosis models with auto grafting method. The successfully modeled rats were randomly divided into six groups, including model group, Yangxue Huayu Xiaozheng decoction high-dose group, middle-dose group, low-dose group (12, 8, 4 g·kg^-1,respectively) and Gestrinone group (0.05 g·kg^-1), n=10 in each group. After ig administration respectively for 28 days, the lesion tissue samples of rats were collected, and the inhibitory effect of Yangxue Huayu Xiaozheng decoction on endometriosis tissue in rats was observed. The expression of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor1 (VEGFR-1) were detected in ectopic endometrium by using RT-PCR and Western blot technique. Result: Compared with the normal group, the expression of VEGF and VEGFR-1 mRNA and protein in endometriosis tissues of rats in model group was significantly higher (P<0.05). Compared with the model group, endometriosis tissues of rats in drug groups were significantly inhibited, and the expression of VEGF and VEGFR-1 mRNA and protein in endometriosis tissues of rats in various drug groups was significantly lower than that in model group (P<0.05). Conclusion: Yangxue Huayu Xiaozheng decoction could obviously inhibit the development of endometriosis tissues in model rats. It may have a closely relation with the inhibition of VEGF/VEGFR signaling pathway to regulate the angiogenesis of lesions.