KGM组方抗炎抗过敏药效评价及作用机制的网络药理学预测

目的 评价KGM组方的抗炎抗过敏药效，并对其潜在的作用机制进行预测。方法 利用透明质酸酶活性抑制试验和二甲苯致小鼠耳肿胀模型试验评价KGM的抗炎抗过敏作用，运用网络药理学预测其起效机制。结果 KGM对透明质酸酶的抑制率为41%，对小鼠耳肿胀抑制率约为44%，表明其具有一定的抗炎抗过敏作用。网络药理学预测结果显示，KGM主要组成成分作用于炎症、过敏及免疫相关靶点209个；绘制成分-靶点-疾病相互作用网络图并结合GO与KEGG富集分析发现，GBA、AKR1B1、CASP3、VDR、CA2、CA1、CA12、CA9、ACE、FUCA1、MGAM与SI等是KGM的主要作用靶点，涉及的细胞功能主要包括G蛋白偶联受体信号通路调节、分泌调节、离子输运调节等；涉及的通路主要包括G蛋白偶联受体信号通路、氮代谢、钙离子通道、淀粉和蔗糖代谢、cAMP信号通路等。结论 KGM具有一定的抗炎抗过敏作用，其机制可能与调控G蛋白偶联受体信号通路、钙离子通道、cAMP信号通路等有关。

Study on anti-allergic efficacy of KGM and its mechanism prediction based on network pharmacology

Objective To evaluate the anti-allergic effect of KGM, and predict the potential mechanism.Methods hyaluronidase activity inhibition experiment and xylene induced ear swelling model experiment were used to verify the anti-allergic effect of KGM in vivo and in vitro, network pharmacology was used to predict its mechanisms of action. Results The inhibitory rate of KGM on hyaluronidase was 41%, and the inhibitory rate of KGM on ear swelling of mice was 44%, which indicated that KGM had some anti-inflammatory and allergic effects. Additionally, the network pharmacology prediction results showed that there are 209 potential targets of the main components of KGM acting on inflammation, allergy and immunity. Through drawing the component-target-disease interaction network map and analyzing the results of GO and KEGG, GBA, AKR1B1, CASP3, VDR, CA2, CA1, CA12, CA9, ACE, FUCA1, MGAM and SI and so on are the main effect targets of KGM, and the cellular functions associated with the targets mainly include G protein-coupled receptor signaling pathway regulation, secretion regulation, ion transport regulation and so on. The pathways associated with the targets mainly include G protein-coupled receptor signaling pathway, nitrogen metabolism, calcium channel, starch and sucrose metabolism, cAMP signaling pathway, and so on. Conclusion KGM had some anti-inflammatory and antiallergic effects, and its mechanism may be related to the regulation of G protein-coupled receptor signaling pathway, calcium channel and cAMP signaling pathway and so on.