通络散结汤对2型糖尿病大鼠调节性T细胞及炎症因子的影响

目的:探讨通络散结汤对高脂饲料联合链脲佐菌素诱导2型糖尿病大鼠模型调节性T细胞及炎症因子的影响.方法:以链脲佐菌素诱导SD大鼠复制2型糖尿病模型,并将造模成功的30只大鼠随机分为三组,即模型组(M组)、通络散结汤高剂量(10 g/kg)组(S组),通络散结汤低剂量(5 g/kg)组(T组),每组10只,同时以10只未造...

Effects of Tongluo Sanjie decoction on T regulatory cells and inflammatory factors in type 2 diabetic rats

Objective:To investigate the effect of Tongluo Sanjie decoction on regulatory T cells and inflammatory factors in a rat model of type 2 diabetes induced by high-fat diet combined with streptozotocin.Methods:SD rats were induced to replicate type 2 diabetes models with streptozotocin,and 30 rats successfully modeled were randomly divided into 3 groups,namely model group(M group),Tongluo Sanjie decoction high-dose(10 g/kg)group(S group),Tongluo Sanjie decoction low-dose(5 g/kg)group(T group),10 rats in each group,and 10 unmodeled rats constitute a normal control group(N group).After modeling,each dose group of Tongluo Sanjie decoction was given corresponding doses of drugs by gavage,M and N groups were given equal volume of normal saline by gavage;12 h after the last administration,blood was collected from the abdominal aorta of each group.Detect FBG,TC,TG,TNF-αand IL-6 in serum.The expression levels of inflammatory factors such as CRP and T lymphocytes such as CD 4⁺and CD 8⁺.At the same time,rat spleen tissues were taken,and western blotting was used to detect the expression levels of CD 4⁺,CD 8⁺and other T lymphocyte proteins.Results:Compared with the M group,the serum levels of FBG,TC,and TG in the N group increased significantly,the levels of inflammatory factors such as TNF-α,IL-6,and CRP increased significantly,and the expression of CD 4⁺T lymphocytes increased significantly,The expression of CD 8⁺T lymphocytes was significantly reduced,the ratio of CD 4⁺/CD 8⁺was significantly increased,and the differences between the groups were statistically significant(all P<0.05);Compared with the N group,the serum FBG,TC,TG,etc.of the rats in the S and T groups The level of inflammatory factors such as TNF-α,IL-6,and CRP was significantly reduced.The expression of CD 4⁺T lymphocytes was significantly reduced,the expression of CD 8⁺T lymphocytes was significantly increased,and the CD 4⁺/CD 8⁺ratio was significantly reduced.The differences were statistically significant(all P<0.05).Conclusion:Tongluo Sanjie decoction has a therapeutic effect on type 2 diabetic rats induced by high-fat diet combined with streptozotocin,and its mechanism may be related to the regulation of inflammatory factors and T cell levels.