高效液相色谱波长切换法同时测定参芪益气口服液中3种成分含量

目的建立HPLC波长切换法同时测定参芪益气口服液中绿原酸、阿魏酸、毛蕊异黄酮葡萄糖苷含量的方法。方法采用反相高效液相法,色谱柱为Agilent HC-C18(250 nm×4.6 nm,5μm),流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,流速1.0 ml/min,采用波长转换法,绿原酸、毛蕊异黄酮葡萄糖苷检测波长为260 nm,阿魏酸为327 nm。结果绿原酸、阿魏酸、毛蕊异黄酮葡萄糖苷线性范围分别为70.40~704.00μg(r=0.9998)、1.36~13.60μg(r=0.9998)、1.28~12.80μg(r=0.9997),平均加样回收率分别为99.44%(RSD=2.54%)、101.06%(RSD=1.57%)、98.00%(RSD=2.09%)。结论所建立的HPLC法方便、简便、准确,适用于同时对参芪益气口服液中绿原酸、阿魏酸及毛蕊异黄酮葡萄糖苷的含量测定。

Determination of three components in Shenqi-Yiqi oral liquid with HPLC combined with switching wavelengthmethod

Objective To establish the high performance liquid chromatography(HPLC)combined with switching wavelength method to determinate chlorogenic acid,ferulic acid,Calycosin-7-glucoside in Shenqi-Yiqi oral liquid.Methods The Shenqi-Yiqi oral liquid was separated on an Agilent HC-C18 column by gradient elution using acetonitrile(A)-0.1%phosphate(B)as the mobile phase.The flow rate was 1.0 ml/min.The detection wavelengths were 260 nm(chlorogenic acid and Calycosin-7-glucoside),316 nm(ferulic acid)by wavelength conversion method.Results The linear ranges of chlorogenic acid,ferulic acid,Calycosin-7-glucoside were 70.40-704.00μg(r=0.9998),1.36-13.60μg(r=0.9998),1.28-12.80μg(r=0.9997),the average recovery rate were 99.44%(RSD=2.54%),101.06%(RSD=1.57%),and 98.00%(RSD=2.09%).Conclusion This method is convient,rapid and accurate for determinating chlorogenic acid,ferulic acid and Calycosin-7-glucoside in Shenqi-Yiqi oral liquid.