结直肠癌RAS、BRAF基因状态与临床病理特征的关系

目的 分析中国人群结直肠癌患者中KRAS、NRAS及BRAF基因突变状态与临床病理特征之间的关系.方法 回顾性分析2018年1月—2020年11月于福建省肿瘤医院接受治疗的370例结直肠癌患者的肿瘤组织标本,检测KRAS、NRAS、BRAF基因突变状态及MMR蛋白表达状态,分析基因突变状态与结直肠癌临床病理特征的关系....

Relationship Between RAS,BRAF Gene Status and Clinicopathological Characteristics of Colorectal Cancer

Objective To analyze the relationship between the mutation status of KRAS,NRAS and BRAF gene and clinicopathological characteristics in colorectal cancer patients in Chinese population.Methods The tumor tissue samples of 370 colorectal cancer patients treated in Fujian cancer hospital from January 2018 to November 2020 were retrospectively analyzed.The mutation status of KRAS,NRAS,BRAF and the expression status of MMR protein were detected,and the relationship between the mutation status and the clinicopathological characteristics of colorectal cancer was analyzed.Results Among the 370 patients,there were 155(41.9%)mutations in KRAS gene,19(5.1%)mutations in NRAS gene,and 40(10.8%)mutations in BRAF gene,among which one patient had G469V mutation and the others had V600E mutation.The mutation rate of KRAS gene was higher in female and high school differentiated patients(P<0.05).NRAS gene mutations were more common in poorly differentiated,invasive patients(P<0.05).BRAF V600E mutation has a high mutation rate in patients with right colon cancer,less than 50 years of age,poorly differentiated,invasive,lymph node,and distant metastasis(P<0.05).Conclusion KRAS gene mutation is related to gender and degree of differentiation,NRAS gene mutation is related to degree of differentiation and general classification of tumor,and BRAF gene V600E mutation is related to primary site,age,degree of differentiation,general classification of tumor,regional lymph node and distant metastasis.KRAS,NRAS and BRAF genes were detected in colorectal cancer patients to provide theoretical basis for understanding the distribution of hot gene mutations in Chinese patients and clinical accurate diagnosis and treatment.