| HPLC法测定流感茶中葛根素的含量 |
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| 引用本文： | 林颖怡,廖晓嘉,周燕双. HPLC法测定流感茶中葛根素的含量[J]. 中国现代药物应用, 2008, 2(9): 3-4 |
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| 作者姓名： | 林颖怡 廖晓嘉 周燕双 |
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| 作者单位： | 广州王老吉药业股份有限公司,510450 |
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| 摘 要： | 目的采用HPLC法测定流感茶中葛根素的含量。方法采用高效液相色谱法，流动相为甲醇-水（25：75），检测波长为250nm。结果葛根素在7．841-250．9μg范围里呈较好的线性关系；葛根素的回收率为99．15％。结论方法操作简单，结果可靠，重复性好，为流感茶质量控制提供科学依据。
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| 关 键 词： | 流感茶 葛根素 高效液相色谱法 |
Determination of puerarin in Liugan Cha by HPLC |
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| LIN Ying-yi,LIAO Xiao-jia,ZHOU Yan-shuang. Determination of puerarin in Liugan Cha by HPLC[J]. Chinese Journal of Modern Drug Application, 2008, 2(9): 3-4 |
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| Authors: | LIN Ying-yi LIAO Xiao-jia ZHOU Yan-shuang |
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| Affiliation: | LIN Ying-yi ,LIAO Xiao-jia ,ZHOU Yan-shnang( Guangzhou wanglaoji Pharrnaceutical imited Company, Guangzhou 510450 , China ) |
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| Abstract: | Objective To establish a HPLC method for determining the contents of Puerarin in Liugan Cha. Method An HPLC method was developed, mobile phase： methanol-water （ 25 ： 75 ） , wavelength at 250 nm. Results Linearity was found in the range of 7. 841-250. 9 μg, the average recovery was 99. 15%. Conclusion This result show that This method is easy to handle , accurate and with good reproducibility , it can be used for quality control for Liugan Cha. |
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| Keywords: | Liugan Cha Puerarin HPLC |
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