| HPLC-ELSD法测定贞芪扶正片黄芪甲苷的含量 |
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| 引用本文： | 吴杏梅,廖华卫,蔡先东,陈树新,陈翠环.HPLC-ELSD法测定贞芪扶正片黄芪甲苷的含量[J].中国现代药物应用,2008,2(10):1-2. |
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| 作者姓名： | 吴杏梅 廖华卫 蔡先东 陈树新 陈翠环 |
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| 作者单位： | 1. 广东省佛山市第一人民医院,528000
2. 广东药学院中药学院 |
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| 摘 要： | 目的建立测定贞芪扶正片中黄芪甲苷含量的高效液相色谱-蒸发光散射检测器法（HPLC-ELSD）。方法采用Kromasil C18（250mm×4.6mm,5μm）的色谱柱;以甲醇-水（80∶20）为流动相;流速：1ml/min;ELSD检测条件：检测器漂移管温度为80℃,氮气压力为50psi,柱温为30℃。结果黄芪甲苷在1.5225-15.225μg范围内与峰面积分别取自然对数后呈良好的线性关系（r=0.9994,n=6）,平均回收率是99.98%（RSD=0.3%）。结论HPLC-ELSD法测定贞芪扶正片中黄芪甲苷含量简便、准确、可靠。
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| 关 键 词： | 高效液相色谱-蒸发光散射检测器法 贞芪扶正片 黄芪甲苷 |
Determination of Astragaloside IV in Zhenqi fuzheng tablets by HPLC-ELSD |
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| Institution: | WU Xing-mei,LIAO Hua- wei, CAI Xian-dong et al.( The First Hospitalof Foshan, Foshan, Guangdong, 528000 China) |
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| Abstract: | Objective To determine astragaloside Ⅳ in zhenqi fuzheng tablet by HPLC-ELSD. Methods HPLC-ELSD was used in the quantitative analysis of astragaloside Ⅳ in the sample. The separationwas performed on Kromasil C18 （250 mm × 4.6 mm, 5 μm） column with a mixture of methanol-water（ 80： 20） as mobile phase and the temperature of column was 30℃. The flow rate was 1.0 ml/min. The temperature of drift tube for measurement was 80℃, and the flowrate of N2 was 50psi. Results The standard curve was linear in the range of 1. 522 5-15.22 5 μg for astragaloside Ⅳ （r =0. 999 4,n =6）. The average recovery was 99. 98% and RSD was 0. 3 %. Conclusion The method is simple, reliable, and is a good method to determinate astragaloside Ⅳ. |
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| Keywords: | HPLC-ELSD qizhenfuzheng tablet astragaloside Ⅳ |
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