六神丸中麝香酮和胆酸的含量测定

目的:测定六神丸中麝香酮和胆酸的含量,考察六神丸中天然麝香和牛黄的质量。方法:采用气相色谱法测定麝香酮含量,色谱条件:采用HP50+石英毛细管色谱柱(30m×0.53mm,1μm),火焰离子化检测器(FID),检测器:190℃,以N2为载气,柱温为165℃,进样口为150℃。采用高效液相色谱法测定胆酸含量,色谱条件:以十八烷基硅烷键合硅胶为填充剂;以乙腈-0.15%磷酸水溶液(39:61)为流动相;检测波长为192nm。结果:麝香酮和胆酸分别在23.90～1195.00μg/mL(r=0.9997,n=6)和0.20512～4.1024mg/mL(r=0.9999,n=11)范围内呈良好线性关系,平均回收率分别为99.64%(RSD=2.00%,n=6)和98.25%(RSD=1.06%,n=6)。结论:2种方法都能准确测定六神丸中麝香酮和胆酸的含量,其结果可一定程度上体现六神丸中天然麝香和牛黄的质量。

Determination of Muskone and Cholalic Acid in Liushen Pill

Purpose：To assay muskone and cholalic acid in Liushen Pill for the quality investigation of Liushen Pill. Method：HPLC method was used to assay cholalic acid, in which C18 was used for the separation, aeetonitrile-0.15% phosphate acid solution （39：61） was used as mobile phase, and the detective wavelength was at 192nm.GC method was used to assay muskone, in which HP 50＋ capillary column was selected for the separation （30 m 0.53 mm, 1μm）, FID was used as detector with the temperature set at 190℃, cartier was N2, the column temperature was 165℃, and the inlet was at 150℃. Result：muskone and cholalic acid fell in the range of 23.90- 1195.00 μg/mL（r = 0.9997, n=6） and 0.20512-4.1024 mg/mL （r=0.9999, n=11） respectively, the average recoveries were 99.64%{RSD= 2.00%, n=6） and 98.25% （RSD=1.06%,n=6}. Conclusion ：Two methods can accurately determine muskone and cholalic acid in Liushen Pill, the determination result could reflect the quality of Liushen Pill to some extent.