| 西罗莫司高产菌株与野生菌株中FKBP25的初步比较 |
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| 引用本文： | 陈纯,林风,黄捷,陈崇宏,程元荣. 西罗莫司高产菌株与野生菌株中FKBP25的初步比较[J]. 中国药理学通报, 2002, 18(5): 579-583 |
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| 作者姓名： | 陈纯 林风 黄捷 陈崇宏 程元荣 |
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| 作者单位： | 1. 福建医科大学药学系药理学教研室,福州,350004
2. 福建省微生物研究所,福州,350007 |
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| 摘 要： | 目的 对比西罗莫司高产突变菌株———吸水链霉菌R2 7、R388、R4 4 1和野生菌株———吸水链霉菌ATCC 36 7817的蛋白质电泳图谱及FKBP2 5含量的差异 ,探讨FKBP2 5与西罗莫司产量的可能关系。方法 制备、分离高产突变株与野生菌株的菌体蛋白质并进行PPIase活性测定 ,比较 3株西罗莫司高产突变株与野生菌株的蛋白质电泳图谱及PPI ase酶活性。结果 西罗莫司低产的野生菌株的FKBP2 5含量比西罗莫司高产突变菌株高。结论 初步认为在吸水链霉菌中西罗莫司的产量与FKBP2 5量的多寡呈现一定的相关性
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| 关 键 词： | 西罗莫司 FK506结合蛋白 肽酰-脯氨酰顺-反异构酶 吸水链霉菌 |
| 文章编号： | 1001-1978(2002)05-0579-05 |
| 修稿时间： | 2002-01-30 |
The preliminary comparison of FKBP25 in the higher sirolimus producer mutants and wild strain |
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| CHEN Chun,LIN Feng ,HUANG Jie ,CHEN Chong Hong,CHENG Yuan Rong. The preliminary comparison of FKBP25 in the higher sirolimus producer mutants and wild strain[J]. Chinese Pharmacological Bulletin, 2002, 18(5): 579-583 |
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| Authors: | CHEN Chun LIN Feng HUANG Jie CHEN Chong Hong CHENG Yuan Rong |
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| Affiliation: | CHEN Chun,LIN Feng 1,HUANG Jie 1,CHEN Chong Hong,CHENG Yuan Rong 1 |
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| Abstract: | AIM To investigate the possible relationship between the yield of sirolimus and the FKBP25 in streptomyces hygroscopicus . METHODS The mycelial proteins and PPIase activities were compared between the three higher sirolimus producer mutants and wild strain. RESULTS The quantity of FKBP25 in wild strain was higher than that in all three mutant stains. CONCLUSION The initial results showed that there may be a relevance between the yield of sirolimus and the quantity of FKBP25 in S hygroscopicus. |
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| Keywords: | sirolimus FKBPs PPIase streptomyces hygroscopicus |
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