| 用CL法和微量MPO法观察人参皂甙对人PMN吞噬杀菌作用 |
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| 引用本文： | 张力平,沈海中,程松高.用CL法和微量MPO法观察人参皂甙对人PMN吞噬杀菌作用[J].中华微生物学和免疫学杂志,1994(2). |
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| 作者姓名： | 张力平 沈海中 程松高 |
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| 作者单位： | 首都医科大学微生物学教研室 |
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| 基金项目： | 中国科学院自然科学基金 |
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| 摘 要： | 采用化学发光（ＣＬ）法和髓过氧化物酶（ＭＰＯ）微量测定法检测了人参茎叶皂甙对人多形核白细胞（ＰＭＮ）吞噬金黄色葡萄球菌的影响。实验结果表明，人参茎叶皂甙作用过的ＰＭＮ－ＣＬ反应（ＣＬ３０和Ｐｅａｋ）和ＭＰＯ释放水平均增强，对ＣＬ３０与ＣＦＵ、ＭＰＯ与ＣＦＵ行直线相关分析均呈密切相关（ｒ＝０．９５８，ｐ＜０．００１；ｒ＝０．８７５，ｐ＜０．０１）。本实验提示，ＰＭＮ－ＣＬ法和ＭＰＯ微量测定法是研究药物对ＰＭＮ吞噬杀菌功能影响的客观、定量和快速的方法。
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| 关 键 词： | 多形核白细胞，化学发光，髓过氧化物酶，人参茎叶皂甙 |
INVESTIGATION OF GINSENOSIDE ON HUMAN PMN PHAGOCYTOSIS BY CL AND MPO
MICROMEASUREMENT |
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| Zhang Li-ping,Shen Hai-zhong and Cheng Son-gao.INVESTIGATION OF GINSENOSIDE ON HUMAN PMN PHAGOCYTOSIS BY CL AND MPO
MICROMEASUREMENT[J].Chinese Journal of Microbiology and Immunology,1994(2). |
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| Authors: | Zhang Li-ping Shen Hai-zhong and Cheng Son-gao |
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| Abstract: | The effect of ginsenoside on
human polymorphonuclear leukocytte(PMN)phagocytosis and killing function wasevaluated by
chemiluminescence(CL ) assay and myeloperoxidase(MPO )release micromeasurement. The
resultsshowed that the CL response(CL30 and Peak )and MPO release level of
ginsenoside-pretreated PMN were en-hanced.The CL30 was closely related to CFU of remainder
S.aureus(r=0.875,P<0.01),the MPO release levelwas also closely related to CFU
r=0.958,P<0.001),The experiment indicated that the CL assay and MPO re-lease
micromeasurement were the quantitative ,objective and rapid method for evaluating the
phagocytosis andkilling function of PMN effected by the medicines . |
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| Keywords: | Polymorphonuclear leukocyte(PMN) Chemiluminescence(CL) Myeloperoxidase(MPO) Ginsenoside |
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