鼠抗人血管抑素单克隆抗体的制备和鉴定

目的:制备鼠抗人血管抑素(angiostatin)的单克隆抗体,为进一步研究血管抑素的抗肿瘤作用和临床应用打下基础。方法:利用杂交瘤技术以血管抑素免疫BALB/C小鼠制备3株能够稳定分泌抗血管抑素抗体的杂交瘤,并对上清进行ELISA、双向免疫扩散等鉴定。结果:该抗体的类别为IgG₁,并能特异性识别血管抑素,与细胞因子rhIL-2、rhTNF-α、rhIFN-α及血清蛋白无交叉反应。结论:经初步鉴定获得3株分泌特异性抗血管抑素抗体的杂交瘤。

Preparation and identification of anti - angiostatin monoclonal antibodies

AIM:To further study the anti-tumor effect of angiostatin, an anti-human angiostatin monoclonal antibody was prepared and identified.METHODS:The hybrodoma techniques were used. The BALB/C mice were immunized with angiostatin. The supernatant of cell culture were collected and screened by ELISA and double immunodiffusion.RESULTS:There cell lines which steadily secreted the anti-angiostatin monoclonal antibody were identified by ELISA and double immunodiffusion. The antibody was IgG1 and specifically recognized angiostatin without crossing reactions to rhIL-2, rhTNF-α, rhIFN-α and serum proteins.CONCLUSION:The antibodies secreted by three hybridoma cell lines identified by several methods were specific antibodies of angiostatin.