癫痫持续状态后神经原损伤与耐药基因产物P-gp关联性研究

目的研究癫痫发作后神经元损伤与耐药基因产物P-gp的关系,探讨难治性癫痫与神经元损伤的关联性。方法采用氯化锂-匹罗卡品法制备癫痫持续状态大鼠动物模型,随机分为健康对照组、癫痫持续状态（SE）后6h、12h、1d、3d、5d及7d组。采用图像分析法进行神经原损伤的病理学研究;流式细胞仪检测血液中P-gp表达阳性细胞率;应用Pearson相关性统计法得出癫痫发作后神经原损伤与P-gp表达的相关性。结果癫痫持续状态导致神经原损伤,3-7天最明显;相应时间点P-gp表达也同步增高;神经原损伤严重程度与P-gp表达呈正相关。结论难治性癫痫与癫痫发作后神经原损伤相关。

Association of neuronal injury following status epilepsy and expression of P-glycoprotein

Objective To study the association of neuronal injury following status epilepsy and expression of P-glycoprotein,discuss the association of intractable epilepsy and neuronal injury.Methods To establish a mice status epilepticus model by lithium-pilocarpine injected intraperitoneal,The mice were randomly divided into control group,6 h、12 h、1 d、3 d、5 d and 7 d group after status epilepticus（SE）.To research the neuronal injury by image analysis method,expression of P-gp in blood was studied by flow cyometry.To analysis the association between neuronal injury and expression of P-gp by Pearson statistics.Results The neuronal injury was observed following status epilepsy,neuronal injury is serious at 3-7day after status epilepsy,expression of P-gp synchronized increased,the severity of neuronal injury has a positive correlation with expression of Pgp.Conclusion There is association between the intractable epilepsy and neuronal injury.