制备小鼠病毒性心肌炎模型最适柯萨奇病毒B3浓度的实验研究

目的寻找制备病毒性心肌炎模型的最适病毒浓度,为筛选治疗病毒性心肌炎药物提供实验模型。方法采用柯萨奇病毒B(3CVB3)Nancy株,经腹腔注射,感染BALB/C小鼠。按注射病毒浓度的不同将75只小鼠分为4组:A组20只,注射高浓度CVB(3104TCID50);B组20只,注射中等浓度CVB(3102TCID50);C组20只,注射低浓度CVB(3100TCID50);D组15只,作为正常对照组。接种后第7d随机从各组取5只小鼠,眼眶取血后处死,称重,并留取心脏标本,将剩余小鼠喂养至第21d,然后将存活小鼠全部处死。观察4组小鼠的发病特征、死亡率、心脏组织病理学变化、心脏重量/身体重量(HW/BW)比值以及血清肌酸激酶同工酶(CK-Mb)水平。结果A组小鼠症状明显较其他3组严重,死亡率达100.0%,高于其他3组,B组死亡率显著高于C组;第7d,A组HW/BW比值、心肌病理评分以及CK-Mb水平均明显高于其他3组,B组HW/BW比值、心肌病理评分以及CK-Mb水平均显著高于C组。结论小鼠的死亡率、心肌病理评分以及CK-Mb水平与接种病毒浓度密切相关,本实验制备病毒性心肌炎小鼠模型最适病毒浓度为102TCID50。

Study on optimal virus concentration to establish the murine viral myocarditis model of Coxsackievirus B3

Objective To investigate the dosage of Coxsackievirus B3 for establishing murine myocarditis model. Methods BALB/C mice were intraperitoneally inoculated with different dilution of CVB3. Seventy five mice were divided into 4 groups: high dosage group (104TCID50),middle dosage group (102TCID50),lower dosage group (100TCID50),and control group. The mortality rate,myocardial histopathology changes,heart to body weight (HW/BW) ratio and serum myocardial enzyme were examined. Results The mortality rate in high dosage group was significantly higher than that of other groups (100.0%,55.0%,10.0% and 0,respectively).The myocardial lesions,HW/BW ratio and serum myocardial enzyme at d7 in the high dosage group were significantly higher than those of other groups. The middle dosage group showed a significantly increased myocardial lesions,HW/BW ratio and serum myocardial enzyme compared with the lower dosage group. Conclusion The 102TCID50 inoculation concentration is suggested to establish the murine myocarditis model.