| 三羟基异黄酮诱导食管癌EC-109细胞凋亡的研究 |
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| 引用本文： | 吴婵妮,周海波,陈金明. 三羟基异黄酮诱导食管癌EC-109细胞凋亡的研究[J]. 浙江医学, 2008, 30(12) |
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| 作者姓名： | 吴婵妮 周海波 陈金明 |
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| 作者单位： | 1. 萧山医院
2. 浙江大学医学院附属第二医院消化科,杭州,310009 |
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| 摘 要： | 目的探讨三羟基异黄酮诱导食管癌EC-109细胞发生凋亡的可能性,揭示该凋亡发生与bcl-2和bax之间的关系。方法采用MTT比色法测定三羟基异黄酮对EC-109细胞的生长抑制率;以透射电镜和TUNEL染色法研究三羟基异黄酮与EC-109细胞凋亡的关系;通过免疫组织化学法和RT-PCR法检测bcl-2和bax的表达。结果三羟基异黄酮对EC-109细胞有明显抑制作用,随浓度增加和培养时间的延长而增强;三羟基异黄酮诱导EC-109细胞出现凋亡细胞形态;TUNEL染色法可见,经三羟基异黄酮处理24、48、72、96h后,EC-109细胞凋亡数随时间明显增加。免疫组织化学发现经处理24、48、72、96h后,EC-109细胞的bcl-2蛋白阳性率减少,bax蛋白阳性率增加。RT-PCR也发现经处理24、48、72、96h后,EC-109细胞的bcl-2mRNA条带密度降低,bax mRNA条带密度加强。结论三羟基异黄酮可能通过下调bcl-2的表达和上调bax的表达而诱导食管癌EC-109细胞发生凋亡。
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| 关 键 词： | 三羟基异黄酮 食管癌 细胞凋亡 |
Apoptosis induced by genistein in esophageal cancer EC-109 cells and its relation to Bcl-2 and Bax |
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| WU Chenni,ZHUO Haibo,CHEN Jinming. Apoptosis induced by genistein in esophageal cancer EC-109 cells and its relation to Bcl-2 and Bax[J]. Zhejiang Medical Journal, 2008, 30(12) |
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| Authors: | WU Chenni ZHUO Haibo CHEN Jinming |
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| Affiliation: | WU Channi,ZHUO Haibo,CHEN Jinming.Department of gastroenterology,The Second offiliated Hospital,Zhejiang University school of Medicine,Hangzhou 310009,China |
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| Abstract: | Objective To investigate the effect of genistein in induction of apoptosis of esophageal cancer EC-109 cells and its relation to the expression of Bcl-2 and Bax.Methods MTT assay was used to determine the cell growth inhibitory rate.Transmission electron microscope and TUNEL staining method were used to detect cell apoptosis of before and after the genistein treatment.Immunohistochemical staining and RT-PCR was used to detect the expression of Bcl-2 and Bax.Rusults Genistein inhibited the growth of esophage... |
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| Keywords: | Genistein Esophageal cancer Apoptosis |
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