大鼠肝癌模型的化学诱导及肝癌肝移植模型的建立

目的 探讨大鼠肝癌模型的化学诱导方法和肝癌肝移植模型建立的手术操作技巧。方法 采用二乙基亚硝胺（diethylnitrosamine，DENA）诱发大鼠肝癌；在Kamada用“二袖套法”吻合血管的基础上、进行改良，并重建大鼠肝动脉血供，建立肝癌大鼠原位肝移植（OLT）模型。结果 诱癌18周以后，诱发肝癌组大鼠行肝移植和不行肝移植两组的1个月存活率分别为56．3％和21．4％，其差异有统计学意义（x^2=5．129，P〈0．05）。大鼠肝癌肝移植实验组手术成功率为87．5％，肝移植对照组手术成功率为90．0％；肝癌肝移植实验组中，移植术后肿瘤的复发和转移多表现为肺部转移和腹腔转移，而移植肝肿瘤复发仅2例；肝癌肝移植实验组和肝移植对照组的两组累积生存率差异有统计学意义。结论 DENA诱发肝癌的方法能大批量诱发出相对稳定的肝脏肿瘤；肝癌肝移植可以明显提高肝癌大鼠生存率；重建肝动脉血供的大鼠原位肝移植模型的是一个更符合生理和肿瘤演进的OLT模型。

Induction of chemical hepatocarcinogenesis and establishment of orthotopic liver transplantation model with hepatocarcinoma in rats

Objective To explore the inducing method for hepatic carcinoma in rats and the operative technique in establishment of orthotopic liver transplantation(OLT) model in rats with induced hepatic tumor.Methods Hepatic carcinoma was induced by diethylnitrosamine(DENA) in rats.Then OLT was performed,using a two-cuff vessel anastomosis method modified from that introduced by Kamada,with reconstruction of hepatic artery.Results At the observation time point(18 weeks) after the initiation of carcinogenesis,the one-month survival rate in OLT group was higher than that in non-OLT group,56.3% and 21.4% respectively,P<0.05.The operative successful rate in tumor rats group and control group was 87.5% and 90.0%,respectively.In OLT rats with hepatic carcinoma group,metastasis mainly occurred in lung and abdomen,while only 2 cases of intrahepatic recurrence were observed.There was significant difference between the tumor rats group and control group in cum survival rate.Conclusion DENA can wholesale induce stable rat model for hepatic carcinoma.OLT can markedly elevate survival rate in rats with liver cancer.In rats with induced hepatic tumor,OLT model,suggested by the author,was established with hepatic arterial reconstruction and it offers a satisfied method with more similar pattern of physiological setting and carcinoma development situation.