放创复合伤皮肤伤口成纤维细胞凋亡变化及其机理研究

目的 研究合并全身放射损伤的创伤（放创复合伤）局部成纤维细胞凋亡变化及其机理。方法 采用组织学、免疫组化、透射电镜、原位凋亡检测等方法观察大鼠皮肤伤口局部成纤维细胞数量、凋亡变化及调亡调控分子Bcl-2和Bax含量变化。结果 放创复合伤皮肤伤口成纤维细胞凋亡较单纯创伤组显著增加，伤口局部Bax含量也显著增加，Bcl-2含量和Bcl-2/Bax比值较单纯创伤组显著降低。结论 全身放射损伤可使创伤局部成纤维细胞凋亡增加，从而使伤口局部成纤维细胞数量减少；伤口局部Bcl-2、Bax含量及其比值异常可能是成纤维细胞凋亡增加的重要原因。

Apoptosis of dermal fibroblasts in wounds combined with radiation injury and its mechanisms

Objective To study apoptosis of dermal fibroblasts in wounds combined with total body irr adiaation (TBI) injury and its mechanisms. Methods We determined the amount of dermal fibroblasts in wounds of rats using histological method,observed apoptosis of dermal fibroblasts in wounds by transmission electron microscope and TUNEL assay,and detected the expression of Bcl|2 and Bax in wounds with immunohistochemistry method. Results The ratio of fibroblastic apoptosis in wounds combined with TBI injury significantly increased as compared with that of simple incision injury.The contents of Bax in wounds combined with TBI injury were significantly higher than those of simple incision injury,and the contents of Bcl|2 and Bcl|2/Bax ratio in the fomer were significantly lower. Conclusion Apoptosis of dermal fibroblasts in wounds combined with TBI injury increases significantly,which causes reduction of the amount of fibroblasts.Abnormalities of Bcl|2,Bax and Bcl|2/Bax ratio are important reasons of the increase of fibroblastic apoptosis.