调节肠道菌群和预防肠道菌群失调动物模型探讨

目的建立调节肠道菌群动物模型和预防肠道菌群失调动物模型。方法(1)调节肠道菌群动物模型小鼠灌服抗生素溶液，小鼠肠道菌群失调后，随机选取1组以生理盐水灌胃，作为自然恢复组，其余3组分别灌服微生态调节剂(主要成份为双歧杆菌，蜡样芽胞杆菌)，20％低聚果糖及中药(含党参、白术、茯苓、甘草等)，给受试物后无菌采取粪便，观察各组双歧杆菌，乳杆菌，肠杆菌及肠球菌数量。(2)预防肠道菌群失调动物模型小鼠灌服抗生素溶液同时分别加生理盐水，微生态调节剂，20％低聚果糖及中药(同前)，给受试物后无菌采取粪便，观察各组双歧杆菌，乳杆菌，肠杆菌及肠球菌数量。结果(1)调节肠道菌群动物模型菌群失调后，给予微生态调节剂，果糖及中药后，小鼠肠道内的双歧杆菌，乳杆菌及肠杆菌在小鼠肠道内的数量与自然恢复组相比较明显增加；(2)预防肠道菌群失调动物模型给予微生态调节剂，果糖及中药后，与正常对照组相比较小鼠肠道内的双歧杆菌，乳杆菌及肠杆菌的数量明显增加。结论微生态调节剂，果糖及中药可产生调节小鼠肠道菌群，增殖双歧杆菌和乳杆菌的作用。

Establishment of Models of Intestinal Flora Regulation and Intestinal Flora Imbalance Prevention in Mice

Objective To establish animal models of intestinal flora regulation and intestinal flora imbalance prevention. Methods 1 The animal model of intestinal flora regulation:40 mice used in this experiment were randomly divided into 4 groups, each of 10 mice. Antibiotics were used for 3 days to create intestinal flora imbalance in mice. Then the mice of control group received saline and the mice in other 3 experimental groups received microecosystem regulator, fructose and traditional Chinese medicine, respectively, for 5 days for intestinal floras regulation. Feces samples of the mice were taken by aseptic manipulation and cultured for assessment of amount of enterobacteriaceae, bifidobacterium and lactobacillus floras growing in the intestine. 2 The model of intestinal flora imbalance prevention: 40 mice were randomly divided into 4 groups, each containing 10 mice. Along with the antibiotics, the mice in the 4 groups received saline, microecosystem regulator, fructose and traditional Chinese medicine, respectively, for 5 days. Samples of feces of the mice were taken after the completion of experiment and the amount of bacterial floras were assessed with the same procedure as in the first experiment. Results 1 The model of intestinal flora regulation:Compared with the control group using saline, all the 3 experimental groups showed that bifidobacterium, lactobacillus floras and intestinal bacillus were significantly increased after treatment with the microecosystem regulator, fructose and traditional Chinese medicine, respectively. 2 The model of intestinal flora imbalance prevention: Compared with the control group using normal saline, the bifidobacterium, lactobacillus floras and intestinal bacillus were significantly increased in the 3 experimental groups after treatment with microecosystem regulator, fructose and traditional Chinese medicine, respectively. Conclusion The microecosystem regulator, fructose and traditional Chinese medicine may excert effects to prevent the intestinal flora imbalance and increase the bifidobacterium, lactobacillus and intestinal bacillus floras in the intestine.