IL-13基因多态性位点rs20541和rs1800925单核苷酸多态性 与儿童支气管哮喘关联性的研究

目的:研究IL-13基因多态性位点rs20541和rs1800925单核苷酸多态性与儿童支气管哮喘发病的关联性.方法:应用聚合酶链反应(PCR)、等位基因特异性PCR(AS-PCR)和直接测序方法检测105例儿童哮喘患者和112名正常儿童的IL-13基因上rs20541和rs1800925单核苷酸多态性分布特点.结果:第4外显子中的+2044G→A(rs20541)哮喘组与正常对照组呈现明显差异(P<0.05).而-1112C→T(rs1800925)位点两组间差异无统计学意义(P>0.05).结论:+2044G→A(rs20541)与哮喘患儿的发病具有强烈相关性.-1112C→T无明显相关性.

Association Between IL-13 Gene rs20541 and rs1800925 Single Nucleotide Polymorphism and Asthma in Children

Objective:To evaluate the association between the IL-13 gene rs20541 and rs1800925 single nucleo tide polymorphisms and asthma in children.Methods:Polymerase chain reaction(PCR),allele-specific PCR(AS-PCR),and direct sequencing were used to detect the IL-13 gene rs20541 and rs1800925 single nucleotide polymorphism distribution in 105 cases of pediatric asthma and 112 normal children.Results:Of the +2044 G→ A(rs20541)site in the exon 4,the patient and normal control groups showed significant differences(P<0.05).No significant difference in the-1112C→T(rs1800925)site was noted between the 2 groups.Conclusion:The rs20541 site has a strong correlation to the occurrence of asthma,while no significant correlation was seen in rs1800925.