手足口病患儿血清IL-6、IL-10、IL-17水平的变化及其临床意义

 手足口病患儿血清IL-6、IL-10、IL-17水平的变化及其临床意义黄小霏1，檀卫平1*，江润昌2 摘要：【目的】探讨手足口病(Hand，foot and mouth disease，HFMD)患儿外周血白介素-6（IL-6）、白介素-10（IL-10）、白介素-17（IL-17）水平及其在HFMD发展过程中的变化。【方法】本实验采用酶联免疫吸附实验（ELISA）双抗体夹心法测定19例HFMD患儿（急性期、恢复期）及同期20例健康体检儿童血清IL-6、IL-10、IL-17的含量；并对HFMD患儿急性期、恢复期及健康儿童进行两两比较，了解IL-6、IL-10、IL-17在HFMD发展过程中的变化。【结果】1、手足口病患儿急性期IL－6、 IL－10及IL－17水平显著高于健康对照组（P<0.01）；恢复期IL－6及IL－17水平与对照组比较无显著差异（P>0.05）。而IL－10水平仍高于健康对照组（P<0.01）。2、急性期IL-6与IL-10呈负相关关系(P<0.05)；IL-6与IL-17呈正相关关系(P<0.01)；IL-10与IL-17呈负相关关系(P<0.05)。恢复期：IL-6与IL-10呈负相关关系(P<0.05)；IL-6与IL-17呈正相关关系(P<0.01)；IL-10与IL-17无相关关系(P>0.05)。【结论】手足口病患儿急性期炎症细胞因子（IL-6、IL-17）水平显著升高，可能参与HFMD的发生、发展过程；而抑制性细胞因子IL-10水平升高持续到恢复期，可能与手足口病患儿恢复后持续存在免疫功能低下有关。关键词：手足口病 儿童 白介素

Change and Clinical Significance of Serum IL-6,IL-10,and IL-17 level in Children with Hand,Foot,and Mouth Disease

【Objective】 To study the changes and clinical significance of serum interleukin(IL)-6,IL-10,and IL-17 levels in the children with hand foot and mouth disease(HFMD).【Method】 The serum concentration of IL-6,IL-10,and IL-17 in 19 cases of HFMD children(acute stage and convalescence) and 20 cases of healthy controls were measured by ELISA.The cytokines levels were compared.【Results】 1.Compared with healthy controls,the levels of IL-6,IL-10,and IL-17 in acute stage of HFMD were significantly increased in HFMD children(P < 0.01).There was no significant difference of the levels of IL-6 and IL-17 between the convalescence of HFMD and healthy children(P > 0.05),However the levels of IL-10 in convalescence of HFMD were higher than healthy children(P < 0.01).2.Spearman correlation analysis revealed that IL-6 and IL-17 were negatively correlated with IL-10(P < 0.05);IL-6 positively correlated with and IL-17 in acute stage(P < 0.01).In convalescence IL-6 was negatively correlated with IL-10(P < 0.05),positively correlated with IL-17(P < 0.01);IL-10 and IL-17 have no correlative relation(P > 0.05).【Conclusions】 1.The levels of IL-6 and IL-17 were significantly increased in acute stage of HFMD children.2.The persistent elevation of serum IL-10 level was found in HFMD patients after convalescence.