结肠癌组织和癌旁组织miRNA表达谱研究

目的探讨结肠癌癌组织和癌旁组织中miRNA的表达差异，寻找潜在的结肠癌特异表达谱。方法miRNA表达芯片检测手术切除未发生转移的原位结肠癌组织和癌旁组织标本中miRNA表达水平，并采用Real-time PCR对差异表达的miRNA进行验证。结果miRNA表达芯片分析发现肿瘤细胞中24种miRNA表达下调，27种表达上调。miR-145、miR-143在癌组织中表达显著下调，miR-451在癌组织中表达显著上调，并被Real-time PCR方法进一步证实。结论结肠癌癌组织和癌旁组织中miRNA的表达存在差异，miR-145、miR-143在结肠癌组织中表达下调，miR-451表达上调，结肠癌组织具有特异miRNA表达谱，可作为结肠癌潜在诊断芯片进行开发。

microRNA Expression Profile between Colon Cancer Tissues and Noncancerous Tissues Surrounding Colon Cancer

Objective To profile the miRNA expression pattern between colon cancer tissues and noncancerous tissues surrounding colon cancer.Methods The expression level of miRNAs in tumor and adjacent normal tissue from clinical colon cancer patients without metastasis was detected by Chip-array.The expression level of miRNA was measured by Real-time PCR.Results Down-regulation of 24 miRNAs and up-regulation of 27 miRNAs were identified in tumor tissue compared with adjacent normal tissue.The expression level of miR-145 and miR-143 was significantly down-regulated in tumor tissue,while the expression level of miR-451 was increased in tumor tissue.All results were validated by Real-time PCR.Conclusion The expression profile of miRNA is different between tumor tissue and adjacent normal tissue.Expression level of miR-145 and miR-143 is significantly down-regulated in colorectal tumor,while expression level of miR-451 is increased.