再植牙复合牙周膜细胞的实验研究

目的:研究体外培养的牙周膜细胞与牙根共同再植入人造牙槽窝内,其组织形成的能力与促进牙周再生的潜能。方法:拔除2只杂种狗的第三、第四前磨牙,得到牙周膜细胞并进行体外培养。拔牙后1个月,将体外培养的牙周膜细胞与自体牙根共同再植入右侧下颌无牙区的人造牙槽窝内,左侧下颌作为对照。再植2个月后,处死动物,标本制成石蜡切片,HE染色,光镜下进行组织学观察。结果:实验组标本中,在与近根尖部分牙根相对的一些区域可以看到垂直于骨表面的纤维束包埋于牙槽窝壁中。结论:即使被植入人造牙槽窝中,体外培养的牙周膜细胞仍保留了在体内形成类牙周膜组织的能力。

An experimental study of tooth root replantation combined with in vitro cultured periodontal ligament cells

Objective:To test the periodontal regeneration potential after tooth root replantation combined with in vitro cultured periodontal ligament cells (PDLCs).Methods:The third and fourth mandibular premolars of two mongrel dogs were extracted and the dog PDLCs were in vitro cultured.The autologous roots combined with PDLCs were replanted into the created cavities in the right mandibular edentulous area one month after premolar extraction.Roots replanted without PDLCs on the left mandible served as the controls. The dogs were sacrificed 2 months after the replantation. Paraffin sections were made and stained with hematoxylin & eosin, then examined histologically.Results:In the samples of root combined with PDLCs implantation, some fiber bundles vertical to the surface of bone were embedded in some inner walls of the alveolar socket opposite to the apical root.Conclusion:Cultured PDLCs in vitro retain the capacity of PDL-like tissue formation even in the artificial alveolar socket.