| Endometrial Mucin-1 and Pinopode in Peri-implantation Phase in Ovarian High Responders during Controlled Ovarian Hyperstimulation Cycles |
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| 引用本文： | Qiu-ju CHEN Xiao-xi SUN Lu LI Xiao-hong GAO Yu WU Li-nan CHENG. Endometrial Mucin-1 and Pinopode in Peri-implantation Phase in Ovarian High Responders during Controlled Ovarian Hyperstimulation Cycles[J]. 生殖与避孕(英文版), 2007, 18(3): 187-196. DOI: 10.1016/S1001-7844(07)60023-6 |
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| 作者姓名： | Qiu-ju CHEN Xiao-xi SUN Lu LI Xiao-hong GAO Yu WU Li-nan CHENG |
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| 作者单位： | Qiu-ju CHENa，Xiao-xi SUNa，Lu LI，Xiao-hong GAO，Yu WU，Li-nan CHENG Department of Reproductive Medicine，International Peace Maternity and Child Health Hospital，Shanghai 200030，China |
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| 摘 要： | Objective To investigate effects of ovarian high response on endometrial mucin-1 (MUC1) and pinopode in peri-implantation phase in controlled ovarian hyperstimulation (COH) cycles. Methods Ovarian high response was defined as serum E2 > 15 000 pmol/L on the day of hCG administration in COH cycle using GnRH agonist and recombinant FSH (n=8). Healthy and fertile women were used as the natural control (n=10). Endometrial biopsies were performed on the day of LH 7/hCG 7. Pinopode formation was observed by scanning electron microscope. Expression of MUC1 was detected with quantitative Real-time PCR and immunohistochemistry. Results In high response group, the lumen surface was covered with variant pinopodes and microvillous. The expression of MUC1 mRNA in high response group was lower than that in the natural control (P<0.05). Immunostaining for MUC1 protein in glandular and luminal epithelium in high response group was lower than that in the natural control (P<0.05). Conclusion Asynchronized pinopode appearance and lower expression of MUC1 during peri-implantation period were the characteristics of endometrium in high response group, which may provide a clue of decreased endometrial receptivity in the supraphysiological hormone milieu.
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| 关 键 词： | 子宫内膜 卵巢 高响应 粘液素 |
| 收稿时间： | 2006-09-04 |
| 修稿时间： | 2006-09-04 |
Endometrial Mucin-1 and Pinopode in Peri-implantation Phase in Ovarian High Responders during Controlled Ovarian Hyperstimulation Cycles |
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| Qiu-ju CHEN,Xiao-xi SUN,Lu LI,Xiao-hong GAO,Yu WU,Li-nan CHENG. Endometrial Mucin-1 and Pinopode in Peri-implantation Phase in Ovarian High Responders during Controlled Ovarian Hyperstimulation Cycles[J]. Journal of Reproduction and Contraception, 2007, 18(3): 187-196. DOI: 10.1016/S1001-7844(07)60023-6 |
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| Authors: | Qiu-ju CHEN Xiao-xi SUN Lu LI Xiao-hong GAO Yu WU Li-nan CHENG |
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| Affiliation: | Qiu-ju CHEN(Department of Reproductive Medicine,International Peace Maternity and Child Health Hospital Shanghai 200030,China)；Xiao-xi SUN(Department of Reproductive Medicine,International Peace Maternity and Child Health Hospital Shanghai 200030,China)；Lu LI(Department of Reproductive Medicine,International Peace Maternity and Child Health Hospital Shanghai 200030,China)；Xiao-hong GAO(Department of Reproductive Medicine,International Peace Maternity and Child Health Hospital Shanghai 200030,China)；Yu WU(Department of Reproductive Medicine,International Peace Maternity and Child Health Hospital Shanghai 200030,China)；Li-nan CHENG(Department of Reproductive Medicine,International Peace Maternity and Child Health Hospital Shanghai 200030,China)； |
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| Abstract: | Objective To investigate effects of ovarian high response on endometrial mucin-1 (MUC1) and pinopode in peri-implantation phase in controlled ovarian hyperstimulation (COH) cycles. Methods Ovarian high response was defined as serum E2 > 15 000 pmol/L on the day of hCG administration in COH cycle using GnRH agonist and recombinant FSH (n=8). Healthy and fertile women were used as the natural control (n=10). Endometrial biopsies were performed on the day of LH 7/hCG 7. Pinopode formation was observed by scanning electron microscope. Expression of MUC1 was detected with quantitative Real-time PCR and immunohistochemistry. Results In high response group, the lumen surface was covered with variant pinopodes and microvillous. The expression of MUC1 mRNA in high response group was lower than that in the natural control (P<0.05). Immunostaining for MUC1 protein in glandular and luminal epithelium in high response group was lower than that in the natural control (P<0.05). Conclusion Asynchronized pinopode appearance and lower expression of MUC1 during peri-implantation period were the characteristics of endometrium in high response group, which may provide a clue of decreased endometrial receptivity in the supraphysiological hormone milieu. |
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| Keywords: | endometrium controlled ovarian hyperstimulation high response mucin-1 pinopode |
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