| 抗PML-RARα反义核酸对NB4细胞生长、细胞周期及细胞凋亡的影响 * |
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| 引用本文： | 陈 烨,缪金明,朱学宏,邵念贤,方智雯,欧阳仁荣. 抗PML-RARα反义核酸对NB4细胞生长、细胞周期及细胞凋亡的影响 *[J]. 中国肿瘤生物治疗杂志, 2000, 7(1): 56-59 |
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| 作者姓名： | 陈 烨 缪金明 朱学宏 邵念贤 方智雯 欧阳仁荣 |
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| 作者单位： | 广州中医药大学第二附属医院中心实验室! 广州510120，广州中医药大学第二附属医院中心实验室! 广州510120，广州中医药大学第二附属医院中心实验室! 广州510120，广州中医药大学第二附属医院中心实验室! 广州510120 |
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| 基金项目： | 国家自然科学基金!（39870944），广东省自然科学基金博士启动基金!（97424）资助 |
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| 摘 要： | 目的：研究能分泌人白细胞介素２的重组卡介苗（ｒＢＣＣ－ＩＬ－２）对小鼠腹腔巨噬细胞体外杀伤活性功能的改变，探讨ｒＢＣＧ－ＩＬ－２对宿主免疫功能的影响。方法：采用基因工程技术和电转化技术构建卡介苗，采用ＭＴＴ法测定小鼠腹腔巨噬细胞体外的杀伤活笥。结果：ｒＢＣＧ－ＩＬ－２组小鼠腹腔巨噬细胞杀伤尖性明显大于ｒＢＣＣ组和对照组，且巨噬细胞的杀伤活性在一周内达到最大。结论：ｒＢＣＣ－ＩＬ－２能提高巨噬细胞的
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| 关 键 词： | 巨噬细胞 肿瘤 杀伤活性 rBCG－IL－2 小鼠 |
| 收稿时间： | 1999-01-06 |
| 修稿时间： | 1999-03-02 |
Effect of Recombinant BCG Secreting Human IL-2 on Cytotoxicity of Murine Peritoneal Macrophages |
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| Chen Ye,Miao Jinming,Zhu Xuehong,Shao Nianxian,Fang Zhiwen and Ouyan Rengrong. Effect of Recombinant BCG Secreting Human IL-2 on Cytotoxicity of Murine Peritoneal Macrophages[J]. Chinses Journal of Cancer Biotherapy, 2000, 7(1): 56-59 |
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| Authors: | Chen Ye Miao Jinming Zhu Xuehong Shao Nianxian Fang Zhiwen Ouyan Rengrong |
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| Affiliation: | Shanghai Institute of Hematology, Renji Hospital. Laboratory of Leukemia Research, Shanghai Second Medical University, Shanghai 200001;Shanghai Institute of Hematology, Renji Hospital. Laboratory of Leukemia Research, Shanghai Second Medical University, Shanghai 200001;Shanghai Institute of Hematology, Renji Hospital. Laboratory of Leukemia Research, Shanghai Second Medical University, Shanghai 200001;Shanghai Institute of Hematology, Renji Hospital. Laboratory of Leukemia Research, Shanghai Second Medical University, Shanghai 200001;Shanghai Institute of Hematology, Renji Hospital. Laboratory of Leukemia Research, Shanghai Second Medical University, Shanghai 200001;Shanghai Institute of Hematology, Renji Hospital. Laboratory of Leukemia Research, Shanghai Second Medical University, Shanghai 200001 |
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| Abstract: | Objective To investigate the effect ofrecombinant BCG secreting IL-2 (rBCG-IL-2) on peritoneal macrophages of mouse and inquireinto the effect of rBCG-IL-2 on immunity of host. Methods Gene engineering technology,electricity transference and inoculation tumor cell are adopted; MTT assay is used todetermine tumoricidal effect of macrophages. Results Tumoricidal effect of macrophages ofrBCG-IL-2 groups was higher than those of the control groups and BCG groups, and thetumoricidal effect of macrophages could reach a high state in a week. ConclusionrBCG-IL-2 can raise tumoricidal effect of macrophages, improve immunity of host and is aprospect biological preparation. |
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| Keywords: | recombinant BCG macrophages melanocarcinoma cell strain cytotoxicity |
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