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| 类风湿关节炎成纤维样滑膜细胞体外增殖活性的研究 | |
| 引用本文： | 肖楚吟,潘云峰,吴玉琼,郭兴华,蔡道章.类风湿关节炎成纤维样滑膜细胞体外增殖活性的研究[J].中华关节外科杂志（电子版）,2010,4(5):40-43. |
| 作者姓名： | 肖楚吟 潘云峰 吴玉琼 郭兴华 蔡道章 |
| 作者单位： | 1. 510630,广州,中山大学附属第三医院风湿科;510120,广州医学院第一附属医院风湿科 2. 中山大学附属第三医院风湿科,广州,510630 3. 广州医学院第一附属医院骨科,510120 |
| 摘 要： | 目的研究类风湿关节炎成纤维样滑膜细胞(RA-FLS)的体外增殖活性。方法收集行关节腔镜术或关节置换术的RA、骨关节炎(OA)、关节创伤患者的膝关节滑膜组织标本各3例,应用酶消化法进行FLS的分离培养,采用MTT法比较三种来源细胞的体外增殖活性。结果 RA-FLS在体外培养第3、4、5天时OD值较OA、关节创伤来源者增高(P值均小于0.05)。结论 RA-FLS细胞增殖程度较OA、关节创伤来源者增高。 |
| 关 键 词： | 关节炎 类风湿 细胞增殖 成纤维细胞 滑膜 |
Cell proliferation activity of rheumatoid arthritis fibroblast-like synoviocytes in vitro |
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| XIAO Chu-yin,PAN Yun-feng,WU Yu-qiong,GUO Xing-hua,CAI Dao-zhang.Cell proliferation activity of rheumatoid arthritis fibroblast-like synoviocytes in vitro[J].Chinese Journal of Joint Surgery(Electronic Version),2010,4(5):40-43. | |
| Authors: | XIAO Chu-yin PAN Yun-feng WU Yu-qiong GUO Xing-hua CAI Dao-zhang |
| Institution: | .The Third Affiliated Hospital,Sun Yat-sen,University,Guangzhou 510630,China |
| Abstract: | Objective To investigate cell proliferation activity of rheumatoid arthritis （RA） fibroblast-like synoviocytes （RA-FLS） in vitro.Methods Synovial tissues were obtained during joint replacement surgery or arthroscopy from three patients with RA,three patients with osteoarthritis （OA）,and three patients with joint trauma （Trauma group）.FLS from three groups were isolated and cell proliferation of these three FLS was analyzed by MTT assay.Results The OD values of RA-FLS were found higher than that of OA-FLS and Trauma-FLS at the 3rd day,the 4th day and the 5th day when cultured in vitro.Conclusions Cell proliferation of RA-FLS was found higher than that of OA-FLS or Trauma-FLS. |
| Keywords: | Arthritis rheumatoid Cell proliferation Fibroblasts Synovial membrane |
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