| 半巢式PCR和PCR-RFLP法诊断耐青霉素肺炎链球菌的比较 |
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| 引用本文： | 丁晶晶,张德平,邵海枫,施毅. 半巢式PCR和PCR-RFLP法诊断耐青霉素肺炎链球菌的比较[J]. 临床检验杂志, 2011, 0(2) |
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| 作者姓名： | 丁晶晶 张德平 邵海枫 施毅 |
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| 作者单位： | 南京大学医学院附属鼓楼医院呼吸科;南京军区南京总医院临床中心实验科;南京军区南京总医院呼吸科; |
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| 基金项目： | 江苏省社会发展科技基金(BS2005051); 南京军区医药卫生科研基金(2006MA109) |
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| 摘 要： | 目的评价半巢式PCR和PCR-RFLP法用于诊断肺炎链球菌青霉素耐药性的价值。方法收集130株肺炎链球菌临床分离株和50株健康人定植株,琼脂稀释法测定青霉素最低抑菌浓度(MIC),半巢式PCR、PCR-RFLP法测定对青霉素耐药性。结果健康人定植株均对青霉素敏感,临床分离株青霉素敏感率、中介率、耐药率分别为25.4%、23.1%和51.5%;当MIC为0.25～0.5μg/ml时,半巢式PCR法检测青霉素耐药性的敏感性、特异性、阳性预测值和阴性预测值分别为86.7%、98.6%、86.7%和98.6%;当MIC≥1μg/ml时,对应值分别为96.2%、97.6%、97.4%和96.4%。而PCR-RFLP法诊断中介株的敏感性仅34.2%;诊断耐药株的阳性预测值仅67.5%。结论半巢式PCR法优于PCR-RFLP法,可用于对耐青霉素肺炎链球菌的快速检测;PCR-RFLP敏感性不高,可用于分子流行病学分析。
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| 关 键 词： | 肺炎链球菌 青霉素 耐药性 半巢式PCR PCR-RFLP |
Comparison of seminested-PCR and PCR-RFLP for detection of penicillin-resistant Streptococcus pneumoniae isolates |
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| DING Jing-jing,ZHANG De-ping,SHAO Hai-fenga,SHI Yib. Comparison of seminested-PCR and PCR-RFLP for detection of penicillin-resistant Streptococcus pneumoniae isolates[J]. Chinese Journal of Clinical Laboratory Science, 2011, 0(2) |
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| Authors: | DING Jing-jing ZHANG De-ping SHAO Hai-fenga SHI Yib |
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| Affiliation: | DING Jing-jing1,ZHANG De-ping1,SHAO Hai-feng2a,SHI Yi2b(1.Department of Respiratory Medicine,Drum Tower Hospital,Nanjing University Medical School,Nanjing 210008,2.a.Institute of Medical Laboratory Science,b.Department of Respiratory Medicine,Nanjing General Hospital of Nanjing Military Command of PLA,Nanjing 210002,Jiangsu,China) |
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| Abstract: | Objective To find out a rapid and reliable method to detect penicillin-resistant Streptococcus pneumoniae(PRSP) and its drug-resistance,and evaluate the application of seminested-PCR and PCR-RFLP for this assay.Methods One hundred and thirty clinical S.pneumoniae isolates and 50 isolates from healthy carriers were collected in Nanjing between March 2005 and June 2007.The minimum inhibitory concentration(MIC) to penicillin was measured by agar dilution test.The genome of S.pneumoniae was extracted and amplif... |
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| Keywords: | Streptococcus pneumoniae penicillin resistance seminested-PCR PCR-RFLP |
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