幼虫冰冻切片抗原间接荧光抗体试验诊断旋毛虫病

目的：评价幼虫冰冻切片抗原间接荧光抗体试验（ＩＦＡＴ）诊断旋毛虫病的价值。方法：应用纯净幼虫冰冻切片间接荧光抗体试验检测感染旋毛虫小鼠和旋毛虫病患者血清中的抗旋毛虫抗体,在患者发病后及应用阿苯达唑治疗后不同时间采集血清,以其他寄生虫病患者和健康人血清为对照。结果：１５只小鼠感染旋毛虫后２ｗｋ即可检出抗体,４６７例旋毛虫病患者的抗体阳性率为８６．５％,而丝虫病、包虫病患者各４５例和健康人血清５６份均为阴性。４８份并殖吸虫病患者的交叉反应率为１６．６７％。旋毛虫病患者发病后１ｗｋ的抗体阳性率为７０．２１％,至发病后２ｗｋ、３ｗｋ及４ｗｋ则分别升至９１．０％、９５．８％及１００％。幼虫冰冻切片的抗原活性于－２０℃可保存至５年。旋毛虫病患者治疗后１ｗｋ抗体阳性率从治疗前的８７．５％上升到１００％,血清抗体滴度比治疗前也有明显升高,治疗后１个月的抗体转阴率为２４％,至治疗后４个月达７５％。结论：幼虫冰冻切片间接荧光抗体试验诊断旋毛虫病具有较高的敏感性和特异性,并可用于疗效考核

DIAGNOSIS OF TRICHINOSIS BY INDIRECT FLUORESCENT ANTIBODY TEST WITH TRICHINELLA LARVA SECTION *

AIM: To evaluate the value of indirect fluorescent antibody test (IFAT) in diagnosing trichinosis. METHODS: IFAT using frozen section of the purified Trichinella larvae as antigen was employed to detect anti-Trichinella antibodies in sera from mice infected with Trichinella spiralis and the patients with trichinosis. Sera from patients with trichinosis were collected at different time intervals after onset of the disease and after treatment with albendazole. Sera from patients with other parasitic diseases and healthy individuals were used as control. RESULTS: The anti-Trichinella antibodies were detected in 15 infected mice as early as 2 weeks after infection, and in 86.5% of 467 patients with trichinosis. Sera from patients with filariasis, echinococcosis and healthy controls were all negative. 8 of 48 (16.7%) cases with paragonimiasis showed positive cross reaction. The antibody positive rate of patients with trichinosis was 70.2% one week after onset of disease and increased to 91.0%, 95.8% and 100% at two, three and four weeks after onset, respectively. The frozen section of the larvae can be stored at -20 degrees C for 5 years without loss of antigenicity. The IFAT positive rate was increased from 87.5% before treatment to 100% one week after treatment, and the serum antibody titers were elevated as well. The antibody negative conversion rate of the patients was 24% at one month after teratment and increased to 75% at four months after treatment. CONCLUSION: IFAT using frozen section of the purified Trichinella larvae as antigen is highly sensitive and specific for the diagnosis of trichinosis and evaluation of the therapeutic effect.