游泳训练对胰岛素抵抗大鼠肝脏抵抗素mRNA表达的影响

目的观察游泳训练对胰岛素抵抗大鼠肝脏抵抗素mRNA表达的影响,初步探讨游泳训练改善胰岛素抵抗的作用机制。方法 30只8周龄正常雄性大鼠随机分为正常饮食对照组（n=9）和高脂高糖喂养造模组（n=21）。喂养6周后,将造模成功的18只胰岛素抵抗大鼠再随机分为模型组（n=9）：继续予高脂高糖饮食;运动组（n=9）：高脂高糖喂养加游泳训练。造模6周和干预6周后采血清全自动生化分析仪测空腹血糖（FPG）,放射免疫法测各组小鼠空腹胰岛素（FIN）,计算胰岛素敏感指数（ISI）。干预6周后留取肝脏组织RT-PCR法检测抵抗素mRNA表达水平。结果高脂高糖饲养6周后,造模组的FPG（6.65±0.98）、FIN（31.04±6.57）均高于对照组,其ISI低于对照组（P〈0.01）。6周干预后,与模型组比较,运动组肝脏组织抵抗素mRNA表达均降低（P〈0.05）,ISI升高（P〈0.05）。结论游泳训练改善胰岛素抵抗可能与下调抵抗素的表达有关。

Effect of swimming training on expression of resistin in patients with insulin resistance

Objective To investigate the effect and the mechanisms of swimming training on the expression of resistin in livers of rats with insulin resistance（IR）.Methods Thirty male Sprague-Dawley rats were randomly divided into normal diet group（control group,n=9）and high-fat/ high-sucrose diet group（n=21）.Eighteen insulin resistant model rats 6 week after induced by diet were randomly subdivided into the model group（feeding with high-fat/high-sucrose diet,n=9） and the exercise group（feeding with high-fat/high-sucrose diet combined with swimming training,n=9）.With high fat diet and swimming interventions for six weeks,then the fasting plasma glucose（FPG）and the fasting insulin（FIN）in these rats were measured by automatic biochemical analyzer and radioimmunoassay,respectively.Insulin sensitive index（ISI）was calculated to ascertain the establishment of insulin resistance.The resistin mRNA expression in livers was estimated by RT-PCR six weeks after intervention.Liver histopathological changes were observed by HE staining method.Results Six weeks after feeding with the high-fat/high-sucrose diet,the serum levels of FBG（6.65±0.98）and FINS（31.04±6.57）in high-fat/high-sucrose diet group were significantly higher,but the LnISI level was significantly lower compared with the control group,（P〈0.01）Six weeks after intervention,the expression of resistin in liver tissues of the exercise group was significantly decreased compared with the model group（P〈0.05）.Conclusions Swimming training could markedly improve insulin resistance,which might be related to the decrease of resistin level.