实时荧光RT-PCR法快速检测流感病毒的应用研究

目的应用实时荧光RT-PCR快速检测2009～2012年河池市流感病毒核酸,为流感的预防控制提供科学依据。方法采集河池市哨点医院的流感样病例和暴发疫点现患病例的咽拭子标本,采用实时荧光RT-PCR方法进行流感病毒核酸检测分型。结果以实时荧光RT-PCR法检测2 090份标本,流感病毒核酸阳性801份,总阳性率为38.28%。其中甲型H1N1流感468份,B型126份,季节性流感H1亚型14份,季节性流感H3亚型109份。结论实时荧光RT-PCR法检测流感病毒具快速、操作方便、特异性强、灵敏度高等优点,值得推广应用。

Rapid detection of influenza virus by real-time fluorenscence RT-PCR

Objective To detect influenza virus nucleic acid by real-time fluorenscence RT-PCR and analyze the results of influenza surveillance in Hechi in 2009-2012. Methods Throat swab samples were collected from influenza-like illness （ILl） patients designated hospitals and those infected in prevalent foci in Hechi city. The influenza viral nucleic acid was determined by using real-time fluorenscence RT-PCR. Results Totally 2 090 samples were detected by real-time RT-PCR and 801 samples positive for influenza virus nucleic acid were detected with a total positive rate of 38.28%, including 468 H1N1 A subtype, 126 HINilB subtype, 14 seasonal influenza viruses H1 subtype and 109 seasonal influenza viruses subtype H3. Conclusion Rreal-time fluorenscence RT-PCR is a quick, simple method for detection of influenza virus with high specificity and high sensitivity, worthy of popularization. In the past four years, influenza virus A subtype H1N1, and influenza virus B, seasonal influenza viruses H1 and seasonal influenza viruses H3 were prevalent in Hechi in the past three years.