胆固醇结石病人与正常人胆囊胆汁的蛋白质表达谱比较分析

目的 比较分析胆日同醇结石病人与正常人胆囊胆汁蛋白表达谱的差异,为阐明胆石成因提供实验依据.方法 提取胆固醇结石病人和正常人胆囊胆汁总蛋白,构建相应的双向电泳蛋白质表达谱,应用lmageMaster图像分析软件获得差异蛋白点信息.结果 胆固醇结石病人与正常人胆囊胆汁总蛋白浓度分别为(6.4824±0.3403)mg/ml和(2.3156±0.3064)mg/ml(P<0.01).两组组内蛋白质平均匹配点数分别为465±71和407±85,平均匹配率为71.70%和68.38%,组间269个蛋白质点相互匹配,匹配率为61.70%.胆固醇结石组蛋白表达谱显著差异点34个,16个表达上调,18个表达下调.结论 初步建立了胆固醇结石病人和正常人胆囊胆汁蛋白表达谱.发现两者存在差异蛋白质点,为筛选胆石形成关键调控子提供基础.

Differential analysis of protein profiles of gallbladder bile from patients with cholesterol cholelithiasis and controls

Objective To elucidate the mechanism of formation of cholesterol cholelithiasis, thisstudy is designed to establish two-dimensional polyacrylamide gel electrophoresis system for differenti-al analysis of the proteome of gallbladder bile from cholesterol cholelithiasis patients and control ones.Methods Total proteins of gallbladder bile were extracted from the subjects in two groups and the protein profiles were established by using the immobilized pH gradient two-dimensional electrophoresis respectively. These maps were analyzed by ImageMaster two-dimensional eleetrophoresis analysis software. Results The concentrations of bile proteins from cholesterol cholelithiasis patients and con-trol ones were (6.4824±0.3403)mg/ml and (2.3156±0.3064)mg/ml(P<0.01), respectively. The average matched protein spots were 465±71 and 407±85 in the two groups, respectively. The matched rates were 71.70% and 68.38% correspondingly. There were 269 matched spots in the two reference gels maps and the matched rate was 61.70%. A total of 34 differential protein spots were i-dentified, 16 expressed highly in cholesterol cholelithiasis patients but lowly in the control ones, while 18 expressed just in the opposite way. Conclusion The differential protein profiles of gallbladder bile between cholesterol cholelithiasis patients and controls have been established and several differential protein spots are found. The data may be a basis for further screening the key regulators of formation of cholesterol eholelithiasis.