siRNA-TRF2对人乳腺癌MCF-7细胞增殖的影响

目的 构建表达端粒重复序列结合因子2基因小干扰RNA的腺病毒表达载体（rAd-shRNA-TRF2），探讨其对人乳腺癌MCF-7细胞增殖的影响。方法 根据预实验筛选出的针对人TRF2 mRNA的特异性siRNA靶序列，构建表达siRNA-TRF2的重组腺病毒载体rAd-shRNA-TRF2，转染人乳腺癌MCF-7细胞后，用MTT比色法检测MCF-7细胞增殖情况，流式细胞仪检测细胞周期分布情况。结果 rAd-shRNA-TRF2转染MCF-7细胞后，MCF-7细胞增殖抑制、细胞周期阻滞于G₀/G₁期、增殖指数显著下降。结论 通过RNAi抑制TRF2基因表达进而抑制人乳腺癌MCF-7细胞增殖的方法有望成为肿瘤基因治疗的一个新策略。

Effects of siRNA-TRF2 on Cell Proliferation of Human Breast Cancer Cell Line MCF-7

Objective Constructed recombinant adenovirus vector for the express of small interfering RNA targeting telomeric repeatbinding factor2,i.e.rAd-shRNA-TRF2 and to investi gate the effects of rAd-shRNA-TRF2 on cell proliferation of human breast cancer MCF-7 cells．Methods Constructed recombinant adenovirus vector (rAd-shRNA-TRF2) expressing siRNA -TRF2 was constructed by selecting, siRNA witch can specifically and effectively express TRF2 mRNA based on preliminary experiments.After rAd-shRNA-TRF2 was transfected into MCF-7 cells, the cell growth were detected by MTT assay and the cell cycle was observed by means of flow cytometry．Results The cell proliferation of MCF-7 was inhibited.The cell cycle of MCF-7 was at G₀/G₁ phase and the cell proliferation index(PI) was breakdown significantly after rAd-shRNA-TRF2 was transfected into MCF-7 cells．Conclusion The cell proliferation of MCF-7 is prohibited by inhibiting the TRF2 express,which maybe become a new gene therapy strategy for the tumor treatment.