COX-2蛋白在胃癌组织、胃癌SGC7901、MGC823细胞株中的表达及意义

目的　探讨环氧合酶 2 (COX 2 )在胃癌组织及胃癌培养细胞中表达及分布情况及其与胃癌生物学活性的关系。方法　应用免疫组织化学、免疫细胞化学、Westernblot方法检测COX 2在胃癌组织、胃癌细胞SGC790 1中的表达 ,并探讨其于与胃癌临床病理的关系。结果　胃癌组织中COX 2蛋白的表达率 95 2 % (4 0 4 2 ) ,15例为低表达 ,2 5例为高表达。与正常粘膜细胞比较COX 2在胃癌组织中的表达明显增强 (P <0 0 5 )。COX 2在高、中分化型腺癌的高表达率为 81 8%为 (18 2 2 ) ,在低分化腺癌中阳中高表达率为 4 0 % (8 2 0 ) ,二者比较有显著性差异 (P <0 0 5 )。COX 2的表达与不同性别、年龄、病期及有无淋巴转移的患者间无显著差异。免疫细胞化学及Westernblot证实在胃癌组织及胃癌细胞SGC790 1中有COX 2过表达。结论　COX 2在胃癌组织中、胃癌细胞系SGC790 1中过表达 ,但并不表达在胃癌细胞系MGC80 3中。COX 2蛋白的表达与胃癌细胞的分化有关 ,与年龄、性别、肿瘤大小、肿瘤分期及淋巴转移无关

Study on cyclooxygenase-2 expression and clinical significance in gastric Carcinognsis and human gastric cancer cell lines

Objective To investigate the role of cyclooxygenase 2 expression in gastriccarcinognsis and human gastric cancer cell lines, and the relationship between their expression and gastric biological behavior. Methods Immunehistochemistry, immunocellchemistry and western blot were used to detect the expression of COX 2 in gastric cancer tissue and gastric cancer cell line. Results The over COX 2 expression was found in gastric cancer tissue and gastric cancer cell lines SGC7901. The high expression rate of COX 2 in well. differentiated cancer was 81 8%, in poorly differentiated gastric was 40%, there was significant difference between well and poorly differentiated gastric cancer. Conclusion There was relationship between COX 2 and development and differentiation of gastric cancer.