IL-17对人表皮细胞DNA荧光密度的影响

目的探讨IL-17对人表皮细胞DNA荧光密度的影响。方法将正常人皮肤移植于裸鼠，建立人皮肤一裸鼠嵌合模型60只；按配对设计要求随机分成实验组30只和对照组30只，实验组注射IL-170.1ml（10ng／m1）于裸鼠移植皮肤内，对照组注射0．9％氯化钠0．1ml于裸鼠移植皮肤内，均1次，d，连续使用28d，最后以激光扫描共聚焦显微镜测移植皮肤人表皮细胞DNA荧光密度值。结果IL-17可使人表皮细胞DNA荧光密度值显著增加[实验组（2414．1667±309．4520）μ㎡VS．对照组（1865．8667±244．8843）μ㎡，t=7．6102，P〈0．05]。结论IL-17可促进人表皮细胞增殖过程中DNA含量升高。

Effect of IL-17 on fluorescence density of DNA in human epidermal cells

Objective To explore the impact of IL- 17 on the fluorescence density of DNA in human epidermal cells. Methods The normal human skin was transplanted to nude mice to establish a skin - nude mice chimeric model in 60 mice. According to requirements in matched-pairs design, the mice were randomly divided into a study group and a control group, 30 for each group. In the study group, IL-17 of 0.1 ml （10 ng/ml） was injected into the skin graft, while 0.9% sodium chloride of 0.1 ml was injected into the skin graft once daily for 28 days. The value of DNA fluorescence density of human epidermal ceils was detected with a laser scanning confocal microscope. Results IL-17 could markedly increase the fluorescence density of DNA in skin cells （t = 7.6102, P〈0.05）. Conclusions IL-17 can promote an increase in DNA content during the proliferation of human epidermal cells.